Overview

  • Product name

  • Description

    Rabbit polyclonal to B1R
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human B1R (internal sequence).

  • Positive control

    • Extracts from K562 cells. LOVO cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab75148 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 40 kDa.
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.

Target

  • Function

    This is a receptor for bradykinin. Could be a factor in chronic pain and inflammation.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family. Bradykinin receptor subfamily. BDKRB1 sub-subfamily.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • B1 bradykinin receptor antibody
    • B1BKR antibody
    • B1R antibody
    • BDKR B1 antibody
    • BDKRB 1 antibody
    • BDKRB1 antibody
    • BK 1 receptor antibody
    • BK-1 receptor antibody
    • BKB1R antibody
    • BKR 1 antibody
    • BKR1 antibody
    • BKRB1_HUMAN antibody
    • BRADY B1 antibody
    • BRADYB1 antibody
    • Bradykinin B1 receptor antibody
    • Bradykinin receptor 1 antibody
    • Bradykinin receptor B1 antibody
    see all

Images

  • All lanes : Anti-B1R antibody (ab75148) at 1/500 dilution

    Lane 1 : Extracts from K562 cells
    Lane 2 : Extracts from K562 cells with immunising peptide at 5 µg

    Lysates/proteins at 25 µg per lane.

    Predicted band size: 40 kDa
    Observed band size: 40 kDa
    Additional bands at: 48 kDa, 50 kDa, 82 kDa. We are unsure as to the identity of these extra bands.

  • Immunofluorescence analysis of B1R in LOVO cells using ab75148, at 1/500 dilution, with (right panel) or without (left panel) preincubation with the immunizing peptide.

References

This product has been referenced in:

  • Ghebrehiwet B  et al. Soluble gC1qR is an autocrine signal that induces B1R expression on endothelial cells. J Immunol 192:377-84 (2014). WB . Read more (PubMed: 24319267) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (Healthy and CLL B-cells, SW837 (rectum adenocarcin)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
40 µg
Specification
Healthy and CLL B-cells, SW837 (rectum adenocarcin
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Dr. Elena Kashuba

Verified customer

Submitted Mar 26 2013

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