Key features and details
- Rabbit polyclonal to B1R
- Suitable for: WB, ELISA, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-B1R antibody
See all B1R primary antibodies
DescriptionRabbit polyclonal to B1R
Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human B1R (internal sequence).
- Extracts from K562 cells. LOVO cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab75148 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 40 kDa.|
|ICC/IF||1/500 - 1/1000.|
FunctionThis is a receptor for bradykinin. Could be a factor in chronic pain and inflammation.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family. Bradykinin receptor subfamily. BDKRB1 sub-subfamily.
Cellular localizationCell membrane.
- Information by UniProt
- B1 bradykinin receptor antibody
- B1BKR antibody
- B1R antibody
All lanes : Anti-B1R antibody (ab75148) at 1/500 dilution
Lane 1 : Extracts from K562 cells
Lane 2 : Extracts from K562 cells with immunising peptide at 5 µg
Lysates/proteins at 25 µg per lane.
Predicted band size: 40 kDa
Observed band size: 40 kDa
Additional bands at: 48 kDa, 50 kDa, 82 kDa. We are unsure as to the identity of these extra bands.
Immunofluorescence analysis of B1R in LOVO cells using ab75148, at 1/500 dilution, with (right panel) or without (left panel) preincubation with the immunizing peptide.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab75148 has been referenced in 1 publication.
- Ghebrehiwet B et al. Soluble gC1qR is an autocrine signal that induces B1R expression on endothelial cells. J Immunol 192:377-84 (2014). WB . PubMed: 24319267