Key features and details
- Rabbit polyclonal to B2R
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-B2R antibody
See all B2R primary antibodies
DescriptionRabbit polyclonal to B2R
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
- WB: MCF7 whole cell lysate. IHC-P: Human brain tissue.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab236093 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Predicted molecular weight: 44 kDa.|
|IHC-P||1/100 - 1/300.|
FunctionReceptor for bradykinin. It is associated with G proteins that activate a phosphatidylinositol-calcium second messenger system.
Tissue specificityUbiquitous. Widespread in normal smooth muscle tissue and neurons.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family. Bradikinin receptor subfamily. BDKRB2 sub-subfamily.
Cellular localizationCell membrane.
- Information by UniProt
- B2 antibody
- B2 bradykinin receptor antibody
- B2BKR antibody
Anti-B2R antibody (ab236093) at 1/500 dilution + MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 44 kDa
Paraffin-embedded human brain tissue stained for B2R using ab236093 at 1/300 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236093 has not yet been referenced specifically in any publications.