Key features and details
- Rabbit polyclonal to B3GALT4
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-B3GALT4 antibody
See all B3GALT4 primary antibodies
DescriptionRabbit polyclonal to B3GALT4
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to Human B3GALT4 aa 42-266.
PAPASPGPPLALPRLLIPNQEACSGPGAPPFLLILVCTAPENLNQRNAIR ASWGGLREARGLRVQTLFLLGEPNAQHPVWGSQGSDLASESAAQGDILQA AFQDSYRNLTLKTLSGLNWAEKHCPMARYVLKTDDDVYVNVPELVSELVL RGGRWGQWERSTEPQREAEQEGGQVLHSEEVPLLYLGRVHWRVNPSRTPG GRHRVSEEQWPHTWGPFPPYASGTG
Database link: O96024
- WB: U-87 MG and A549 whole cell lysates; Mouse skeletal muscle tissue lysate. IHC-P: Human lung cancer and skeletal muscle tissues.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab224630 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/500 - 1/5000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).|
FunctionInvolved in GM1/GD1B/GA1 ganglioside biosynthesis.
Tissue specificityHighly expressed in heart, skeletal muscle and pancreas and, to a lesser extent, in brain, placenta, kidney, liver and lung.
PathwayProtein modification; protein glycosylation.
Sequence similaritiesBelongs to the glycosyltransferase 31 family.
Cellular localizationGolgi apparatus membrane.
- Information by UniProt
- 3-galactosyltransferase 4 antibody
- 3-galactosyltransferase antibody
- 3-GalTase 4 antibody
All lanes : Anti-B3GALT4 antibody (ab224630) at 1/500 dilution
Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Lane 2 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 3 : Mouse skeletal muscle tissue lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
Paraffin-embedded human lung cancer tissue stained for B3GALT4 using ab224630 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human skeletal muscle tissue stained for B3GALT4 using ab224630 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab224630 has not yet been referenced specifically in any publications.