Overview

  • Product name

  • Description

    Rabbit polyclonal to B3GAT1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to Human B3GAT1 (internal sequence). NP_061114.
    Database link: Q9P2W7

  • Positive control

    • Mouse brain tissue lysate and Human thyroid cancer tissue.
  • General notes

    Previously labeled as CD57.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.3
    Preservative: 0.05% Sodium azide
    Constituents: 49% PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab199156 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/25 - 1/100.
WB 1/200 - 1/1000. Predicted molecular weight: 38 kDa.

Target

  • Function

    Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl-sphingomyelin was the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group.
  • Tissue specificity

    Mainly expressed in the brain.
  • Pathway

    Protein modification; protein glycosylation.
  • Sequence similarities

    Belongs to the glycosyltransferase 43 family.
  • Cellular localization

    Golgi apparatus membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 3-glucuronyltransferase 1 antibody
    • 3-glucuronyltransferase antibody
    • B3GA1_HUMAN antibody
    • B3gat1 antibody
    • Beta 1 3 glucuronyltransferase 1 antibody
    • Beta-1 antibody
    • Beta-1,3-glucuronyltransferase 1 antibody
    • CD 57 antibody
    • CD57 antibody
    • CD57 antigen antibody
    • Galactosylgalactosylxylosylprotein 3 beta glucuronosyltransferase 1 antibody
    • Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1 antibody
    • GlcAT P antibody
    • GlcAT-P antibody
    • GLCATP antibody
    • GlcUAT P antibody
    • GlcUAT-P antibody
    • GlcUATP antibody
    • Glucuronosyltransferase P antibody
    • HNK 1 antibody
    • HNK1 antibody
    • LEU 7 antibody
    • LEU7 antibody
    • LEU7 antigen antibody
    • NK 1 antibody
    • NK1 antibody
    • UDP GlcUA glycoprotein beta 1 3 glucuronyltransferase antibody
    • UDP-GlcUA:glycoprotein beta-1 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue labeling B3GAT1 with ab199156 at 1/40 dilution.

  • Anti-B3GAT1 antibody (ab199156) at 1/400 dilution + Mouse brain tissue lysate at 40 µg

    Predicted band size: 38 kDa


    Exposure time: 20 seconds


    Gel: 10% SDS-PAGE

References

ab199156 has not yet been referenced specifically in any publications.

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