Overview

  • Product name

    Anti-B3GAT1 antibody [HNK-1 or Leu-7]
    See all B3GAT1 primary antibodies
  • Description

    Mouse monoclonal [HNK-1 or Leu-7] to B3GAT1
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Does not react with: Rat
  • Immunogen

    Tissue, cells or virus corresponding to Human B3GAT1. (Human peripheral blood mononuclear cells).

  • Positive control

    • IHC-P: Human tonsil tissue.
  • General notes

    Previously labeled as CD57.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: PBS, 0.05% BSA
  • Concentration information loading...
  • Purification notes

    Purified from Bioreactor Concentrate.
  • Clonality

    Monoclonal
  • Clone number

    HNK-1 or Leu-7
  • Isotype

    IgM
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab233871 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Primary incubation for 30 minutes at RT.

Target

  • Function

    Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl-sphingomyelin was the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group.
  • Tissue specificity

    Mainly expressed in the brain.
  • Pathway

    Protein modification; protein glycosylation.
  • Sequence similarities

    Belongs to the glycosyltransferase 43 family.
  • Cellular localization

    Golgi apparatus membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 3-glucuronyltransferase 1 antibody
    • 3-glucuronyltransferase antibody
    • B3GA1_HUMAN antibody
    • B3gat1 antibody
    • Beta 1 3 glucuronyltransferase 1 antibody
    • Beta-1 antibody
    • Beta-1,3-glucuronyltransferase 1 antibody
    • CD 57 antibody
    • CD57 antibody
    • CD57 antigen antibody
    • Galactosylgalactosylxylosylprotein 3 beta glucuronosyltransferase 1 antibody
    • Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1 antibody
    • GlcAT P antibody
    • GlcAT-P antibody
    • GLCATP antibody
    • GlcUAT P antibody
    • GlcUAT-P antibody
    • GlcUATP antibody
    • Glucuronosyltransferase P antibody
    • HNK 1 antibody
    • HNK1 antibody
    • LEU 7 antibody
    • LEU7 antibody
    • LEU7 antigen antibody
    • NK 1 antibody
    • NK1 antibody
    • UDP GlcUA glycoprotein beta 1 3 glucuronyltransferase antibody
    • UDP-GlcUA:glycoprotein beta-1 antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded human tonsil tissue stained for B3GAT1 using ab233871 at 4 µg/ml in immunohistochemical analysis.

References

ab233871 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab233871.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up