Anti-B3GAT1 antibody [HNK-1 or Leu-7] - BSA and Azide free (ab212403)

Overview

  • Product name

    Anti-B3GAT1 antibody [HNK-1 or Leu-7] - BSA and Azide free
    See all CD57 primary antibodies
  • Description

    Mouse Monoclonal [HNK-1 or Leu-7] to B3GAT1 - BSA and Azide free
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Does not react with: Rat
  • Immunogen

    Tissue, cells or virus corresponding to Human CD57. Human peripheral blood mononuclear cells.

  • Positive control

    • CCRF-CEM cells; Human lymph node or tonsil tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: 100% PBS
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    ab212403 is purified from Bioreactor Concentrate by Protein A/G.
  • Clonality

    Monoclonal
  • Clone number

    HNK-1 or Leu-7
  • Isotype

    IgM
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab212403 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

for 30 minutes at RT

ICC/IF Use a concentration of 0.5 - 1 µg/ml.

Target

  • Relevance

    CD57 is a carbohydrate epitope that contains a sulfoglucuronyl residue in several adhesion molecules of the nervous system. Is is created by Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1 (B3GAT1) The CD57 antigen is present on subsets of T cells and natural killer cells.
  • Alternative names

    • HKN1 antibody
    • LEU7 antibody

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling CD57 with ab212403 at 4μg/ml.

References

ab212403 has not yet been referenced specifically in any publications.

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