Anti-CD57 antibody [HNK-1 or Leu-7] - BSA and Azide free (ab212403)
Key features and details
- Mouse monoclonal [HNK-1 or Leu-7] to CD57 - BSA and Azide free
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgM
Overview
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Product name
Anti-CD57 antibody [HNK-1 or Leu-7] - BSA and Azide free
See all CD57 primary antibodies -
Description
Mouse monoclonal [HNK-1 or Leu-7] to CD57 - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Does not react with: Rat -
Immunogen
Tissue, cells or virus corresponding to Human CD57. Human peripheral blood mononuclear cells.
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Positive control
- CCRF-CEM cells; Human lymph node or tonsil tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
ab212403 is purified from Bioreactor Concentrate by Protein A/G. -
Clonality
Monoclonal -
Clone number
HNK-1 or Leu-7 -
Isotype
IgM -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab212403 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
for 30 minutes at RT |
Notes |
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IHC-P
Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. for 30 minutes at RT |
Target
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Relevance
CD57 is a carbohydrate epitope that contains a sulfoglucuronyl residue in several adhesion molecules of the nervous system. Is is created by Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1 (B3GAT1) The CD57 antigen is present on subsets of T cells and natural killer cells. -
Alternative names
- HKN1 antibody
- LEU7 antibody
Images
Datasheets and documents
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Datasheet download
References (1)
ab212403 has been referenced in 1 publication.
- Guo N et al. A 34-Marker Panel for Imaging Mass Cytometric Analysis of Human Snap-Frozen Tissue. Front Immunol 11:1466 (2020). PubMed: 32765508