Overview

  • Product name

    Anti-B3GAT1 antibody [NK-1] - BSA and Azide free
    See all B3GAT1 primary antibodies
  • Description

    Mouse monoclonal [NK-1] to B3GAT1 - BSA and Azide free
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Does not react with: Rat
  • Immunogen

    Tissue, cells or virus corresponding to Human B3GAT1. Human peripheral blood mononuclear cells.
    Database link: Q9P2W7

  • Positive control

    • Human spleen, lymph node or tonsil tissue.
  • General notes

    Previously labeled as CD57.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: 100% PBS
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    ab212405 is purified from Bioreactor Concentrate by Protein A/G.
  • Clonality

    Monoclonal
  • Clone number

    NK-1
  • Isotype

    IgM
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab212405 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

for 30 minutes at RT

ICC/IF Use a concentration of 0.5 - 1 µg/ml.

Target

  • Function

    Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl-sphingomyelin was the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group.
  • Tissue specificity

    Mainly expressed in the brain.
  • Pathway

    Protein modification; protein glycosylation.
  • Sequence similarities

    Belongs to the glycosyltransferase 43 family.
  • Cellular localization

    Golgi apparatus membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 3-glucuronyltransferase 1 antibody
    • 3-glucuronyltransferase antibody
    • B3GA1_HUMAN antibody
    • B3gat1 antibody
    • Beta 1 3 glucuronyltransferase 1 antibody
    • Beta-1 antibody
    • Beta-1,3-glucuronyltransferase 1 antibody
    • CD 57 antibody
    • CD57 antibody
    • CD57 antigen antibody
    • Galactosylgalactosylxylosylprotein 3 beta glucuronosyltransferase 1 antibody
    • Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1 antibody
    • GlcAT P antibody
    • GlcAT-P antibody
    • GLCATP antibody
    • GlcUAT P antibody
    • GlcUAT-P antibody
    • GlcUATP antibody
    • Glucuronosyltransferase P antibody
    • HNK 1 antibody
    • HNK1 antibody
    • LEU 7 antibody
    • LEU7 antibody
    • LEU7 antigen antibody
    • NK 1 antibody
    • NK1 antibody
    • UDP GlcUA glycoprotein beta 1 3 glucuronyltransferase antibody
    • UDP-GlcUA:glycoprotein beta-1 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human spleen tissue labeling B3GAT1 with ab212405 at 4μg/ml.

References

ab212405 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab212405.
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