Key features and details
- Mouse monoclonal [NK1] to B3GAT1, prediluted
- Suitable for: IHC-P, IHC-Fr, ICC/IF
- Reacts with: Human
- Isotype: IgM
Product nameAnti-B3GAT1 antibody [NK1], prediluted
See all CD57 primary antibodies
DescriptionMouse monoclonal [NK1] to B3GAT1, prediluted
SpecificityThis antibody is specific to a 110kD human CD57 myeloid associated glycoprotein. It stains mononuclear cells with natural killer activity and neuroendocrine cells and their tumors.
Tested applicationsSuitable for: IHC-P, IHC-Fr, ICC/IFmore details
Species reactivityReacts with: Human
Human peripheral blood mononuclear cells.
- Tonsil. This antibody gave a positive result when used in the following methanol fixed cell lines: U2OS
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.50
Preservative: 0.05% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab82749 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration. Fix with acetone.|
RelevanceCD57 is a carbohydrate epitope that contains a sulfoglucuronyl residue in several adhesion molecules of the nervous system. Is is created by Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1 (B3GAT1) The CD57 antigen is present on subsets of T cells and natural killer cells.
- HKN1 antibody
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ICC/IF image of ab82749 stained U2OS cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab82749 at Undiluted (Used Neat) overnight at +4°C. The secondary antibody (pseudo-colored green) was Dylight® 488 goat anti- mouse (ab98674) IgM preadsorbed, used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
ab82749 staining CD57 in formalin-fixed, paraffin-embedded Human tonsil tissue by Immunohistochemistry. Staining was detected using DAB.
ab82749 has been referenced in 1 publication.
- Fang J et al. Prognostic significance of tumor infiltrating immune cells in oral squamous cell carcinoma. BMC Cancer 17:375 (2017). PubMed: 28549420