Key features and details
- Mouse monoclonal [NK/804] to B3GAT1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgM
Product nameAnti-B3GAT1 antibody [NK/804]
See all B3GAT1 primary antibodies
DescriptionMouse monoclonal [NK/804] to B3GAT1
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Does not react with: Rat
Recombinant fragment corresponding to Human B3GAT1.
Database link: Q9P2W7
- Human tonsil tissue.
Previously labeled as CD57.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA
Concentration information loading...
Purification notesab220187 is purified from Bioreactor Concentrate.
Light chain typekappa
Our Abpromise guarantee covers the use of ab220187 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
for 30 minutes at RT
FunctionInvolved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl-sphingomyelin was the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group.
Tissue specificityMainly expressed in the brain.
PathwayProtein modification; protein glycosylation.
Sequence similaritiesBelongs to the glycosyltransferase 43 family.
Cellular localizationGolgi apparatus membrane.
- Information by UniProt
- 3-glucuronyltransferase 1 antibody
- 3-glucuronyltransferase antibody
- B3GA1_HUMAN antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling B3GAT1 with ab220187 at 4 µg/mL for 30 minutes at room temperature. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at room temperature for 20 minutes.
ab220187 has been referenced in 1 publication.
- Keren L et al. MIBI-TOF: A multiplexed imaging platform relates cellular phenotypes and tissue structure. Sci Adv 5:eaax5851 (2019). Mass Cytometry . PubMed: 31633026