Anti-B3GNT1 antibody (ab236291)

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Overview

  • Product name

    Anti-B3GNT1 antibody

  • Description

    Rabbit polyclonal to B3GNT1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment corresponding to Human B3GNT1 aa 29-397. Lumenal domain.
    Sequence:

    KSSSQEKNGKGEVIIPKEKFWKISTPPEAYWNREQEKLNRQYNPILSMLT NQTGEAGRLSNISHLNYCEPDLRVTSVVTGFNNLPDRFKDFLLYLRCRNY SLLIDQPDKCAKKPFLLLAIKSLTPHFARRQAIRESWGQESNAGNQTVVR VFLLGQTPPEDNHPDLSDMLKFESEKHQDILMWNYRDTFFNLSLKEVLFL RWVSTSCPDTEFVFKGDDDVFVNTHHILNYLNSLSKTKAKDLFIGDVIHN AGPHRDKKLKYYIPEVVYSGLYPPYAGGGGFLYSGHLALRLYHITDQVHL YPIDDVYTGMCLQKLGLVPEKHKGFRTFDIEEKNKNNICSYVDLMLVHSR KPQEMIDIWSQLQSAHLKC


    Database link: Q9NY97

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: HEK-293T, Jurkat and HL-60 whole cell lysates. IHC-P: Human skin tissue.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.40
    Preservative: 0.03% Proclin
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...

  • Purity

    Protein G purified

  • Purification notes

    Purity >95%

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab236291 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/200.
WB 1/500 - 1/2000. Predicted molecular weight: 46 kDa.

Target

  • Function

    Catalyzes the initiation and elongation of poly-N-acetyllactosamine chains.

  • Tissue specificity

    Ubiquitous.

  • Pathway

    Protein modification; protein glycosylation.

  • Sequence similarities

    Belongs to the glycosyltransferase 31 family.

  • Cellular localization

    Golgi apparatus membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • 3-galactosyltransferase 7 antibody
      • 3-GalTase 7 antibody
      • 3-Gn-T1 antibody
      • 3-Gn-T2 antibody
      • 3-N-acetylglucosaminyltransferase 1 antibody
      • 3-N-acetylglucosaminyltransferase 2 antibody
      • b3Gal-T7 antibody
      • B3GN2_HUMAN antibody
      • B3GNT antibody
      • B3GNT2 antibody
      • Beta 1 3 galactosyltransferase 7 antibody
      • Beta-1 antibody
      • Beta-3-Gx-T7 antibody
      • Beta3Gal-T7 antibody
      • Beta3GalT7 antibody
      • Beta3Gn T1 antibody
      • Beta3Gn-T1 antibody
      • Beta3Gn-T2 antibody
      • Beta3GNT antibody
      • BGnT-1 antibody
      • BGnT-2 antibody
      • UDP-Gal:beta-GlcNAc beta-1 antibody
      • UDP-galactose:beta-N-acetylglucosamine beta-1 antibody
      • UDP-GlcNAc:betaGal beta-1 antibody
      see all

    Images

    • Western blot - Anti-B3GNT1 antibody (ab236291)
      Western blot - Anti-B3GNT1 antibody (ab236291)
      All lanes : Anti-B3GNT1 antibody (ab236291) at 1/500 dilution

      Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
      Lane 3 : HL-60 (human promyelocytic leukemia cell line) whole cell lysate

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution

      Predicted band size: 46 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B3GNT1 antibody (ab236291)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B3GNT1 antibody (ab236291)

      Paraffin-embedded human skin tissue stained for B3GNT1 using ab236291 at 1/100 dilution in immunohistochemical analysis.

    References

    ab236291 has not yet been referenced specifically in any publications.

    Publishing research using ab236291? Please let us know so that we can cite the reference in this datasheet.

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