Anti-B4GALT1 antibody - C-terminal (ab175887)

Overview

  • Product name
    Anti-B4GALT1 antibody - C-terminal
    See all B4GALT1 primary antibodies
  • Description
    Rabbit polyclonal to B4GALT1 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Synthetic peptide within Human B4GALT1 aa 336-366 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: P15291

  • Positive control
    • HL60 cells and cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab175887 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 44 kDa.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    The Golgi complex form catalyzes the production of lactose in the lactating mammary gland and could also be responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.
    The cell surface form functions as a recognition molecule during a variety of cell to cell and cell to matrix interactions, as those occurring during development and egg fertilization, by binding to specific oligosaccharide ligands on opposing cells or in the extracellular matrix.
  • Tissue specificity
    Ubiquitously expressed, but at very low levels in fetal and adult brain.
  • Pathway
    Protein modification; protein glycosylation.
  • Involvement in disease
    Defects in B4GALT1 are the cause of congenital disorder of glycosylation type 2D (CDG2D) [MIM:607091]. CDGs are a family of severe inherited diseases caused by a defect in protein N-glycosylation. They are characterized by under-glycosylated serum proteins. These multisystem disorders present with a wide variety of clinical features, such as disorders of the nervous system development, psychomotor retardation, dysmorphic features, hypotonia, coagulation disorders, and immunodeficiency. The broad spectrum of features reflects the critical role of N-glycoproteins during embryonic development, differentiation, and maintenance of cell functions.
  • Sequence similarities
    Belongs to the glycosyltransferase 7 family.
  • Post-translational
    modifications
    The soluble form derives from the membrane forms by proteolytic processing.
  • Cellular localization
    Golgi apparatus > Golgi stack membrane. Cell membrane. Cell surface. Found in trans cisternae of Golgi; Golgi apparatus > Golgi stack membrane. Found in trans cisternae of Golgi and Secreted. Soluble form found in body fluids.
  • Information by UniProt
  • Database links
  • Alternative names
    • 384.Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase antibody
    • 4-galactosyltransferase 1 antibody
    • 4-GalTase 1 antibody
    • b4Gal-T1 antibody
    • B4galt1 antibody
    • B4GT1_HUMAN antibody
    • Beta-1 antibody
    • Beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase antibody
    • Beta4Gal-T1 antibody
    • CDG2D antibody
    • GGTB2 antibody
    • GT1 antibody
    • GTB antibody
    • Lactose synthase A protein antibody
    • N-acetyllactosamine synthase antibody
    • Nal synthase antibody
    • Processed beta-1 antibody
    • UDP-Gal:beta-GlcNAc beta-1 antibody
    • UDP-galactose:beta-N-acetylglucosamine beta-1 antibody
    see all

Images

  • Anti-B4GALT1 antibody - C-terminal (ab175887) at 1/1000 dilution + HL60 cell lysate at 35 µg

    Predicted band size: 44 kDa

  • Flow Cytometric analysis of HL60 cells labeling B4GALT1 with ab175887 at 1/10 dilution (right histogram) compared to a negative control cell (left histogram).  FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

ab175887 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
Sample
Human Cell (293)
Specification
293
Permeabilization
Yes - 0.1% Triton-X
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Mar 09 2015

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (7.5% gel)
Sample
Chinese Hamster Cell lysate - whole cell (ovary cell)
Specification
ovary cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Daniel Wang

Verified customer

Submitted Oct 13 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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