Key features and details
- Rabbit polyclonal to B4GALT3
- Suitable for: WB, ELISA, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-B4GALT3 antibody
See all B4GALT3 primary antibodies
DescriptionRabbit polyclonal to B4GALT3
Specificityab74825 detects endogenous levels of total B4GALT3 protein.
Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthesized peptide derived from an internal region of human B4GALT3
- WB: extracts from HepG2 cells, HeLa cells and Jurkat cells IHC-P: human liver carcinoma tissue
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Our Abpromise guarantee covers the use of ab74825 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 44 kDa.|
|IHC-P||Use at an assay dependent concentration.|
FunctionResponsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.
Tissue specificityFound in various tissues. Highest expression in placenta, prostate, testis, ovary, intestine and muscle, and in fetal brain.
PathwayProtein modification; protein glycosylation.
Sequence similaritiesBelongs to the glycosyltransferase 7 family.
Cellular localizationGolgi apparatus > Golgi stack membrane. Trans cisternae of Golgi stack.
- Information by UniProt
- 4-galactosyltransferase 3 antibody
- 4-galactosyltransferase antibody
- 4-GalTase 3 antibody
All lanes : Anti-B4GALT3 antibody (ab74825) at 1/500 dilution
Lane 1 : extracts from HepG2 cells
Lane 2 : extracts from HeLa cells
Lane 3 : extracts from Jurkat cells
Lane 4 : extracts from Jurkat cells with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 44 kDa
Observed band size: 44 kDa
ab74825, at a dilution of 1/50, staining B4GALT3 in paraffin embedded human liver carcinoma tissue sections by Immunohistochemistry in the absence (left image) or presence (right image) of the immunizing peptide.
ab74825 has been referenced in 1 publication.
- Zhou H et al. B4GALT family mediates the multidrug resistance of human leukemia cells by regulating the hedgehog pathway and the expression of p-glycoprotein and multidrug resistance-associated protein 1. Cell Death Dis 4:e654 (2013). WB ; Human . PubMed: 23744354