Overview

  • Product name
  • Description
    Rabbit polyclonal to B4GALT4
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 103-324 of Human B4GALT4 (Uniprot ID: O60513).

  • Positive control
    • 293T, A431, Jurkat and Raji whole cell lysates. Human H441 xenograft tissue.
  • General notes
    Keep as concentrated solution.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155476 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 40 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).

Target

  • Relevance
    This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. The enzyme encoded by this gene appears to mainly play a role in glycolipid biosynthesis. Two alternatively spliced transcript variants have been found for this gene.
  • Cellular localization
    Golgi apparatus, Golgi stack membrane. Single-pass type II membrane protein. Note=Trans cisternae of Golgi stack.
  • Database links
  • Alternative names
    • B4GALT4 antibody
    • B4Gal T4 antibody
    • B4Gal-T4 antibody
    • Beta 1 4 galactosyltransferase 4 antibody
    • Beta 1 4 GalTase 4 antibody
    • Beta N acetylglucosaminyl glycolipid beta 1 4 galactosyltransferase 4 antibody
    • beta-1,4-galactosyltransferase 4 antibody
    • beta-1,4-GalTase 4 antibody
    • beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase 4 antibody
    • Beta4Gal T4 antibody
    • beta4Gal-T4 antibody
    • EC 2.4.1.- antibody
    • UDP Gal:betaGlcNAc beta ,4 galactosyltransferase 4 antibody
    • UDP Gal:betaGlcNAc beta 1 4 galactosyltransferase polypeptide 4 antibody
    • UDP galactose:beta N acetylglucosamine beta 1 4 galactosyltransferase 4 antibody
    • UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 4 antibody
    • UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase 4 antibody
    • UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 4 antibody
    • UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 4 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human H441 xenograft tissue, labeling B4GALT4 using ab155476 at a 1/250 dilution.
  • Anti-B4GALT4 antibody (ab155476) at 1/1000 dilution + Jurkat whole cell lysate at 30 µg

    Predicted band size: 40 kDa



    10% SDS PAGE

References

ab155476 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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