Key features and details
- Rabbit polyclonal to B7-H6
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-B7-H6 antibody
See all B7-H6 primary antibodies
DescriptionRabbit polyclonal to B7-H6
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Orangutan
Recombinant fragment corresponding to Human B7-H6 aa 25-262.
DLKVEMMAGGTQITPLNDNVTIFCNIFYSQPLNITSMGITWFWKSLTFDK EVKVFEFFGDHQEAFRPGAIVSPWRLKSGDASLRLPGIQLEEAGEYRCEV VVTPLKAQGTVQLEVVASPASRLLLDQVGMKENEDKYMCESSGFYPEAIN ITWEKQTQKFPHPIEISEDVITGPTIKNMDGTFNVTSCLKLNSSQEDPGT VYQCVVRHASLHTPLRSNFTLTAARHSLSETEKTDNFS
Database link: Q68D85
- WB: Mouse heart, liver and brain tissue lysates; HeLa whole cell lysate (ab150035). IHC-P: Human melanoma tissue. ICC/IF: HepG2 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: PBS, 50% Glycerol, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab229999 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/1000 - 1/5000. Predicted molecular weight: 51 kDa.|
|ICC/IF||1/50 - 1/200.|
FunctionTriggers NCR3-dependent natural killer cell activation.
Tissue specificityNot detected in any normal tissue tested. Expressed at the surface of several tumor cell lines including T and B lymphomas, myeloid leukemias, melanomas, carcinomas and large T SV40 antigen-transformed cells (at protein level).
Sequence similaritiesContains 1 Ig-like C1-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
DomainThe C-terminal part is similar to retroviral Gag protein. This putative protein seems to be the result of a fusion between an Ig-like domain-containing protein and a ERV.
- Information by UniProt
- B7 homolog 6 antibody
- B7-H6 antibody
- B7H6 antibody
All lanes : Anti-B7-H6 antibody (ab229999) at 1/1000 dilution
Lane 1 : Mouse heart tissue lysate
Lane 2 : Mouse liver tissue lysate
Lane 3 : Mouse brain tissue lysate
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 51 kDa
Observed band size: 32,38,51 kDa why is the actual band size different from the predicted?
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for B7-H6 (green) using ab229999 at 1/100 dilution in ICC/IF, followed by Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG(H+L) as the secondary antibody.
Paraffin-embedded human melanoma tissue stained for B7-H6 using ab229999 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229999 has not yet been referenced specifically in any publications.