Recombinant Anti-B7-H6 antibody [EPR21841] (ab219205)
- Datasheet
- References
- Protocols
Overview
-
Product name
Anti-B7-H6 antibody [EPR21841]
See all B7-H6 primary antibodies -
Description
Rabbit monoclonal [EPR21841] to B7-H6 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human B7-H6 aa 1-300. The exact sequence is proprietary.
Database link: Q68D85 -
Positive control
- WB: HepG2, Raji, HeLa and K562 whole cell lysates.
-
General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21841 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Related Products
Applications
Our Abpromise guarantee covers the use of ab219205 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 50-80 kDa (predicted molecular weight: 51 kDa). |
Target
-
Function
Triggers NCR3-dependent natural killer cell activation. -
Tissue specificity
Not detected in any normal tissue tested. Expressed at the surface of several tumor cell lines including T and B lymphomas, myeloid leukemias, melanomas, carcinomas and large T SV40 antigen-transformed cells (at protein level). -
Sequence similarities
Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Domain
The C-terminal part is similar to retroviral Gag protein. This putative protein seems to be the result of a fusion between an Ig-like domain-containing protein and a ERV. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 374383 Human
- Omim: 613714 Human
- SwissProt: Q68D85 Human
- Unigene: 146274 Human
-
Alternative names
- B7 homolog 6 antibody
- B7-H6 antibody
- B7H6 antibody
see all
Images
-
![Western blot - Anti-B7-H6 antibody [EPR21841] (ab219205)](https://www.abcam.com/ps/products/219/ab219205/Images/ab219205-314412-anti-b7-h6-antibody-epr21841-western-blot.jpg)
Western blot - Anti-B7-H6 antibody [EPR21841] (ab219205)All lanes : Anti-B7-H6 antibody [EPR21841] (ab219205) at 1/1000 dilution
Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 51 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
MCF7 cells have a low level of B7-H6 expression. The molecular mass observed is consistent with the literature (PMID 24780758).
-
![Western blot - Anti-B7-H6 antibody [EPR21841] (ab219205)](https://www.abcam.com/ps/products/219/ab219205/Images/ab219205-314411-anti-b7-h6-antibody-epr21841-western-blot.jpg)
Western blot - Anti-B7-H6 antibody [EPR21841] (ab219205)All lanes : Anti-B7-H6 antibody [EPR21841] (ab219205) at 1/1000 dilution
Lane 1 : Untreated HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : HepG2 whole cell lysate treated with PNGase F
Lane 3 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 51 kDa
Observed band size: 50-80 kDa why is the actual band size different from the predicted?Exposure time : Lanes 1 and 2: 92 seconds; Lanes 3 and 4: 3 minutes.
Blocking/Dilution buffer: 5% NFDM/TBST.
B7-H6 is a glycoprotein and can be de-glycosylated by PNGase F. The molecular mass observed is consistent with the literature (PMID 24780758).
References
ab219205 has not yet been referenced specifically in any publications.
