Recombinant Anti-B7-H6 antibody [EPR21841] (ab219205)
![Western blot - Anti-B7-H6 antibody [EPR21841] (ab219205)](https://www.abcam.com/ps/products/219/ab219205/Images/ab219205-314412-anti-b7-h6-antibody-epr21841-western-blot.jpg "Western blot - Anti-B7-H6 antibody [EPR21841] (ab219205)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21841] to B7-H6
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-B7-H6 antibody [EPR21841]
See all B7-H6 primary antibodies -
Description
Rabbit monoclonal [EPR21841] to B7-H6 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human B7-H6 aa 1-300. The exact sequence is proprietary.
Database link: Q68D85 -
Positive control
- WB: HepG2, Raji, HeLa and K562 whole cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21841 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab219205 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 50-80 kDa (predicted molecular weight: 51 kDa). |
Target
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Function
Triggers NCR3-dependent natural killer cell activation. -
Tissue specificity
Not detected in any normal tissue tested. Expressed at the surface of several tumor cell lines including T and B lymphomas, myeloid leukemias, melanomas, carcinomas and large T SV40 antigen-transformed cells (at protein level). -
Sequence similarities
Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Domain
The C-terminal part is similar to retroviral Gag protein. This putative protein seems to be the result of a fusion between an Ig-like domain-containing protein and a ERV. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 374383 Human
- Omim: 613714 Human
- SwissProt: Q68D85 Human
- Unigene: 146274 Human
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Alternative names
- B7 homolog 6 antibody
- B7-H6 antibody
- B7H6 antibody
see all
Images
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Western blot - Anti-B7-H6 antibody [EPR21841] (ab219205)All lanes : Anti-B7-H6 antibody [EPR21841] (ab219205) at 1/1000 dilution
Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 51 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
MCF7 cells have a low level of B7-H6 expression. The molecular mass observed is consistent with the literature (PMID 24780758).
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![Western blot - Anti-B7-H6 antibody [EPR21841] (ab219205)](https://www.abcam.com/ps/products/219/ab219205/Images/ab219205-314411-anti-b7-h6-antibody-epr21841-western-blot.jpg)
Western blot - Anti-B7-H6 antibody [EPR21841] (ab219205)All lanes : Anti-B7-H6 antibody [EPR21841] (ab219205) at 1/1000 dilution
Lane 1 : Untreated HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : HepG2 whole cell lysate treated with PNGase F
Lane 3 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 51 kDa
Observed band size: 50-80 kDa why is the actual band size different from the predicted?Exposure time : Lanes 1 and 2: 92 seconds; Lanes 3 and 4: 3 minutes.
Blocking/Dilution buffer: 5% NFDM/TBST.
B7-H6 is a glycoprotein and can be de-glycosylated by PNGase F. The molecular mass observed is consistent with the literature (PMID 24780758).
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![Anti-B7-H6 antibody [EPR21841] (ab219205)](https://www.abcam.com/ps/products/219/ab219205/Images/ab219205-3-benefits-of-recombinant-antibodies.png)
Anti-B7-H6 antibody [EPR21841] (ab219205)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab219205 has not yet been referenced specifically in any publications.
