Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23470-210] to B7-H6
- Suitable for: WB, IP
- Reacts with: Human
Product nameAnti-B7-H6 antibody [EPR23470-210]
See all B7-H6 primary antibodies
DescriptionRabbit monoclonal [EPR23470-210] to B7-H6
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC or IHC-P
Species reactivityReacts with: Human
Recombinant fragment within Human B7-H6 aa 1-300. The exact sequence is proprietary.
Database link: Q68D85
- WB: HepG2 and K562 whole cell lysates. IP: HepG2 whole cell lysate.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab253180 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 50, 80 kDa (predicted molecular weight: 51 kDa).|
FunctionTriggers NCR3-dependent natural killer cell activation.
Tissue specificityNot detected in any normal tissue tested. Expressed at the surface of several tumor cell lines including T and B lymphomas, myeloid leukemias, melanomas, carcinomas and large T SV40 antigen-transformed cells (at protein level).
Sequence similaritiesContains 1 Ig-like C1-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
DomainThe C-terminal part is similar to retroviral Gag protein. This putative protein seems to be the result of a fusion between an Ig-like domain-containing protein and a ERV.
- Information by UniProt
- B7 homolog 6 antibody
- B7-H6 antibody
- B7H6 antibody
All lanes : Anti-B7-H6 antibody [EPR23470-210] (ab253180) at 1/1000 dilution
Lane 1 : Untreated HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : HepG2 whole cell lysate treated with PNGase F 20
Lane 3 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 4 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 51 kDa
Observed band size: 50,80 kDa why is the actual band size different from the predicted?
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
Negative control: MCF7.
The expression profile is consistent with the literature (PMID: 22851709).
B7-H6 is a glycoprotein and can be de-glycosylated by PNGase F. The molecular mass observed is consistent with the literature (PMID: 24780758).
B7-H6 was immunoprecipitated from 0.35 mg HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate with ab253180 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab253180 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate 10 μg
Lane 2: ab253180 IP in HepG2 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab253180 in HepG2 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 41 seconds.
The lower bands are likely to be the deglycosylated form of B7-H6.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab253180 has not yet been referenced specifically in any publications.