Recombinant Anti-B7H4 antibody [BLR043F] (ab243899)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BLR043F] to B7H4
- Suitable for: ICC, IHC-P, IP, WB
- Reacts with: Human
Overview
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Product name
Anti-B7H4 antibody [BLR043F]
See all B7H4 primary antibodies -
Description
Rabbit monoclonal [BLR043F] to B7H4 -
Host species
Rabbit -
Tested applications
Suitable for: ICC, IHC-P, IP, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human B7H4 (extracellular).
Database link: Q7Z7D3 -
General notes
This product is sold under License from Bethyl Laboratories, Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA -
Concentration information loading...
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Purification notes
Recombinant antibody was purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
BLR043F -
Isotype
IgG -
Research areas
Associated products
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab243899 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
1/100 - 1/500.
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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IP |
Use at an assay dependent concentration.
Use 20µl/mg lysate. |
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WB |
1/1000.
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Notes |
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ICC
1/100 - 1/500. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
Use at an assay dependent concentration. Use 20µl/mg lysate. |
WB
1/1000. |
Target
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Function
Negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. Involved in promoting epithelial cell transformation. -
Tissue specificity
Overexpressed in breast, ovarian, endometrial, renal cell (RCC) and non-small-cell lung cancers (NSCLC). Expressed on activated T- and B-cells, monocytes and dendritic cells, but not expressed in most normal tissues (at protein level). Widely expressed, including in kidney, liver, lung, ovary, placenta, spleen and testis. -
Sequence similarities
Belongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 2 Ig-like V-type (immunoglobulin-like) domains. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Cell membrane. Expressed at the cell surface. A soluble form has also been detected. - Information by UniProt
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Database links
- Entrez Gene: 79679 Human
- Omim: 608162 Human
- SwissProt: Q7Z7D3 Human
- Unigene: 546434 Human
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Alternative names
- B7 family member, H4 antibody
- B7 H4 antibody
- B7 homolog 4 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling B7H4 with ab243899 at 1/250 dilution.
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B7H4 was immunoprecipitated from 1.0 mg OVCAR-4 whole cell lysate with ab243899 at 20 µl per reaction Western blot was performed on the immunoprecipitate using ab243899 at 1/1000 dilution.
Lane 1: recombinant rabbit anti-B7H4 monoclonal antibody IP in OVCAR-4 whole cell lysate.
Lane 2: ab243899 IP in OVCAR-4 whole cell lysate.
Lane 3: Control IgG in OVCAR-4 whole cell lysate.
Detection: Chemiluminescence with an exposure time of 3 seconds.
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Western blot analysis using ab243899 at 1/1000 dilution.
Lane 1: OVCAR-4 whole cell lysate (50 µg).
Lane 2: HepG2 whole cell lysate (50 µg).
Lane 3: MCF7 whole cell lysate (50 µg).
Lane 4: HeLa whole cell lysate (50 µg).
Lane 5: HEK-293T whole cell lysate (50 µg).
Lane 6: Jurkat whole cell lysate (50 µg).
Lane 7: MOLT-4 whole cell lysate (50 µg).
Lane 8: HH whole cell lysate (50 µg).
Lane 9: C5/MJ whole cell lysate (50 µg).
A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 3 seconds.
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Formalin-fixed, paraffin-embedded NIH:OVCAR-3 (human ovary adenocarcinoma cell line) cells labeling B7H4 using ab243899 at 1/100 dilution in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining.
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Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for B7H4 using ab243899 at 1/100 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab243899 has not yet been referenced specifically in any publications.