Overview

  • Product name

    Anti-BAALC antibody [7.3_1E10]
    See all BAALC primary antibodies
  • Description

    Mouse monoclonal [7.3_1E10] to BAALC
  • Host species

    Mouse
  • Specificity

    ab127977 specificity has been determined by protein microarray.
  • Tested applications

    Suitable for: WB, IP, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Myeloid Leukemia cells (HL60).

  • Positive control

    • Transfected HeLa cell lysate; Colorectal carcinoma (HCT116) cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab127977 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 19 kDa.
IP Use a concentration of 4 µg/ml. per reaction
ICC/IF 1/100.

Target

  • Function

    May play a synaptic role at the postsynaptic lipid rafts by interacting with CAMK2A.
  • Tissue specificity

    Expressed by neural and hematopoietic cells. Expression is found both in normal early progenitor cells and in the most immature type of blasts in acute leukemia but not in mature hematopoietic cells. Isoform 2 and isoform 6 are expressed in the brain. Isoform 2 shows a low expression in neuroectoderm-derived tissues such as adrenal gland and no expression in bone marrow, peripheral blood lymphocytes or lymph nodes, or in tumors or cancer cell lines of nonneural tissue origin.
  • Post-translational
    modifications

    Palmitoylation and myristoylation target the protein to the lipid rafts.
  • Cellular localization

    Cytoplasm. Membrane. Postsynaptic lipid rafts.
  • Information by UniProt
  • Database links

  • Alternative names

    • BAALC antibody
    • BAALC_HUMAN antibody
    • Brain and acute leukemia cytoplasmic antibody
    • Brain and acute leukemia cytoplasmic protein antibody
    • FLJ12015 antibody
    see all

Images

  • Lanes 1-3 : Anti-BAALC antibody [7.3_1E10] (ab127977) at 1/1000 dilution
    Lanes 4-6 : V5 antibody control at 1/1000 dilution

    Lanes 1 & 4 : Transfected HeLa cell lysate
    Lanes 2 & 5 : Non-Transfected HeLa cell lysate
    Lanes 3 & 6 : SH-SY5Y cell lysate

    Predicted band size: 19 kDa

  • ab127977 at 1/100 staining BAALC in 4% paraformaldehyde fixed, 0.1% Triton X-100 permeabilized HeLa cells by Immunocytochemistry.
  • 2 µg of ab127977 was added to aliquots of cleared cell lysates from transfected Human cell lines and incubated for 2 hr at 4°C. Protein-G Dynabeads were then added, and lysates were incubated for additional 2 hr at 4°C to capture the IgG-protein complex. Anti-V5 antibody was used in parallel as a positive control. As a negative control, primary antibody was excluded.
    Lane 1: Cell Lysate control
    Lane 2: Cell lysate with ab127977
    Lane 3: Cell lysate without ab127977
    Lane 4: V5 tag control

References

ab127977 has not yet been referenced specifically in any publications.

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