• Product name

  • Description

    Rabbit polyclonal to BAAT
  • Host species

  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (IQLTATPVSA LVDEPVHIRA TGLIPFQMVS FQASLEDENG DMFYSQAHYR) of human BAAT (NP_001692).

  • Positive control

    • Human fetal liver lysate



Our Abpromise guarantee covers the use of ab83882 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 46 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.


  • Function

    Involved in bile acid metabolism. In liver hepatocytes catalyzes the second step in the conjugation of C24 bile acids (choloneates) to glycine and taurine before excretion into bile canaliculi. The major components of bile are cholic acid and chenodeoxycholic acid. In a first step the bile acids are converted to an acyl-CoA thioester, either in peroxisomes (primary bile acids deriving from the cholesterol pathway), or cytoplasmic at the endoplasmic reticulum (secondary bile acids). May catalyze the conjugation of primary or secondary bile acids, or both. The conjugation increases the detergent properties of bile acids in the intestine, which facilitates lipid and fat-soluble vitamin absorption. In turn, bile acids are deconjugated by bacteria in the intestine and are recycled back to the liver for reconjugation (secondary bile acids). May also act as an acyl-CoA thioesterase that regulates intracellular levels of free fatty acids. In vitro, catalyzes the hydrolysis of long- and very long-chain saturated acyl-CoAs to the free fatty acid and coenzyme A (CoASH), and conjugates glycine to these acyl-CoAs.
  • Tissue specificity

    Expressed in liver, gallbladder mucosa and pancreas.
  • Involvement in disease

    Defects in BAAT are involved in familial hypercholanemia (FHCA) [MIM:607748]. FHCA is a disorder characterized by elevated serum bile acid concentrations, itching, and fat malabsorption.
  • Sequence similarities

    Belongs to the C/M/P thioester hydrolase family.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ20300 antibody
    • BAAT antibody
    • BAAT_HUMAN antibody
    • BACAT antibody
    • BAT antibody
    • Bile acid CoA: amino acid N-acyltransferase (glycine N-choloyltransferase) antibody
    • Bile acid CoA:amino acid N acyltransferase antibody
    • Bile acid Coenzyme A amino acid N acyltransferase glycine N choloyltransferase antibody
    • Bile acid Coenzyme A: amino acid N acyltransferase antibody
    • Bile acid-CoA:amino acid N-acyltransferase antibody
    • Glycine N choloyltransferase antibody
    • Glycine N-choloyltransferase antibody
    • Long chain fatty acyl CoA hydrolase antibody
    • Long-chain fatty-acyl-CoA hydrolase antibody
    • MGC104432 antibody
    see all


  • Anti-BAAT antibody (ab83882) at 1 µg/ml (in 5% skim milk / PBS buffer) + human fetal liver lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 46 kDa
    Observed band size: 46 kDa


This product has been referenced in:

  • Penno CA  et al. 11ß-Hydroxysteroid dehydrogenase-1 is involved in bile acid homeostasis by modulating fatty acid transport protein-5 in the liver of mice. Mol Metab 3:554-64 (2014). WB ; Mouse . Read more (PubMed: 25061560) »
  • Aleksunes LM  et al. Repression of hepatobiliary transporters and differential regulation of classic and alternative bile acid pathways in mice during pregnancy. Toxicol Sci 130:257-68 (2012). WB ; Mouse . Read more (PubMed: 22903823) »
See all 2 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Western blot
Mouse Tissue lysate - whole (liver)
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Xia Wen

Verified customer

Submitted Jun 23 2012


Thank you for your reply and for submitting your Abreviews. The discount codes I had sent you are now active and can each be used to receive a free antibody on a future order. If you are interested in testing any other antibodies, please let me know and I will be happy to help you further.

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For ab83882: DISCOUNT CODE: ***
For ab97884: DISCOUNT CODE: ***
Expiration date: October 5th, 2012

I am very pleased to hear you would like to accept our offer and test ab83882 and ab97884 in mouse.Each code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for using each antibodymouse and include the appropriatecode in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

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