Western blot analysis of BAAT1 in immunoprecipitation pellets from Human Jurkat lysate. ab181855 used at 1/30 dilution. Secondary antibody Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000. Blocking buffer and concentration: 5% NFDM/TBST.
Flow cytometric analysis of HeLa cells (paraformaldehyde-fixed, 2%) labeling BAAT1 with ab181855 at 1/10 dilution (red) or a rabbit IgG (negative) (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution. Isotype control Rabbit IgG.
Immunofluorescent analysis of 4% paraformaldehyde fixed 293 cells labeling BAAT1 with ab181855 at 1/250 dilution. Secondary antibody Goat anti rabbit IgG(Alexa Fluor®488) at 1/200 dilution, Conterstained with Dapi blue.
Western blot - Anti-BAAT1 antibody [EPR13753] - C-terminal (ab181855)
All lanes : Anti-BAAT1 antibody [EPR13753] - C-terminal (ab181855) at 1/10000 dilution
Lane 1 : HeLa lysate Lane 2 : 293 lysate Lane 3 : Jurkat lysate
Lysates/proteins at 20 µg per lane.
Secondary All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution