Overview

  • Product name
  • Description
    Rabbit polyclonal to BACE1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC, WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to amino acids 485-501 of Human BACE. The peptide sequence differs fromthose of mouse and rat BACE by one amino acid (1).

  • Positive control
    • IHC-P: Mouse brain tissue WB: Human brain tissue lysate, 3T3 lysate. ICC/IF: 3T3 cells
  • General notes

    Beta-site APP Cleaving Enzyme.

Properties

Applications

Our Abpromise guarantee covers the use of ab2077 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa.Can be blocked with BACE1 peptide (ab7883). We recommend overnight incubation at 4C and using 5% skim milk to block.
ICC/IF Use at an assay dependent concentration.
IHC-P Use a concentration of 2.5 µg/ml.

Target

  • Function
    Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase.
  • Tissue specificity
    Expressed at high levels in the brain and pancreas. In the brain, expression is highest in the substantia nigra, locus coruleus and medulla oblongata.
  • Sequence similarities
    Belongs to the peptidase A1 family.
  • Domain
    The transmembrane domain is necessary for its activity. It determines its late Golgi localization and access to its substrate, APP.
  • Post-translational
    modifications
    Glycosylated.
  • Cellular localization
    Membrane. Golgi apparatus > trans-Golgi network. Endoplasmic reticulum. Endosome. Cell surface. Predominantly localized to the later Golgi/trans-Golgi network (TGN) and minimally detectable in the early Golgi compartments. A small portion is also found in the endoplasmic reticulum, endosomes and on the cell surface.
  • Information by UniProt
  • Database links
  • Alternative names
    • APP beta secretase antibody
    • Asp 2 antibody
    • ASP2 antibody
    • Aspartyl protease 2 antibody
    • BACE 1 antibody
    • BACE antibody
    • BACE1 antibody
    • BACE1_HUMAN antibody
    • Beta secretase 1 antibody
    • Beta secretase antibody
    • Beta site amyloid beta A4 precursor protein cleaving enzyme antibody
    • Beta site amyloid precursor protein cleaving enzyme 1 antibody
    • Beta site amyloid precursor protein cleaving enzyme antibody
    • Beta site APP cleaving enzyme 1 antibody
    • Beta site APP cleaving enzyme antibody
    • Beta-secretase 1 antibody
    • Beta-site amyloid precursor protein cleaving enzyme 1 antibody
    • Beta-site APP cleaving enzyme 1 antibody
    • FLJ90568 antibody
    • HSPC104 antibody
    • Memapsin 2 antibody
    • Memapsin-2 antibody
    • Memapsin2 antibody
    • Membrane associated aspartic protease 2 antibody
    • Membrane-associated aspartic protease 2 antibody
    • Transmembrane aspartic proteinase Asp2 antibody
    see all

Images

  • All lanes : Anti-BACE1 antibody (ab2077) at 1 µg/ml

    Lane 1 : Human brain tissue lysate with absence of blocking peptide
    Lane 2 : Human brain tissue
    lysate with BACE1 peptide (ab7883)
    Lane 3 : Mouse 3T3/NIH cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

    Observed band size: 70 kDa
    why is the actual band size different from the predicted?



    Incubate the antibody for 1 hour at room temperature in 5% NFDM/TBST.

     

     

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse 3T3/NIH cells labeling BACE with ab2077 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue). Image showing both membrane and cytosol staining on 3T3/NIH cells.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using ab2077 at 2.5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

  • ab2077 staining BACE1 in human glioblastoma cell line D54MG by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 0.5% BSA for 20 minutes at room temperature, then incubated with ab2077 at a 1/50 dilution for 16 hours at 4°C. The secondary used was a TRITC conjugated goat anti-rabbit polyclonal, used at a 1/400 dilution. Nuclei are counterstained with DAPI.

    See Abreview

  • ab2077 staining BACE1 in rat glioma cell line C6 by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in paraformaldehyde, permeabilized using 0.1% Triton X-100, blocked with 0.5% BSA for 20 minutes at room temperature, then incubated with ab2077 at a 1/50 dilution for 16 hours at 4°C. The secondary used was a TRITC conjugated goat anti-rabbit polyclonal, used at a 1/400 dilution. Nuclei are counterstained with DAPI.

    See Abreview

References

This product has been referenced in:
  • Wu Y  et al. Microglia and amyloid precursor protein coordinate control of transient Candida cerebritis with memory deficits. Nat Commun 10:58 (2019). Read more (PubMed: 30610193) »
  • Satomoto M  et al. Sevoflurane preconditioning ameliorates lipopolysaccharide-induced cognitive impairment in mice. Exp Anim 67:193-200 (2018). Read more (PubMed: 29187700) »
See all 45 Publications for this product

Customer reviews and Q&As

11-15 of 15 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (neuronal)
Loading amount
40 µg
Specification
neuronal
Blocking step
LiCor blocking solution as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Oct 26 2007

Application
Western blot
Sample
Mouse Cell lysate - whole cell (P19 Neuronal Cells)
Loading amount
30 µg
Specification
P19 Neuronal Cells
Treatment
siRNA against BACE-1
Gel Running Conditions
Reduced Denaturing
Blocking step
Serum as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Jul 26 2007

Answer

Thank you for your interest in our antibody products. Unfortunatly, this antibody has only been tested in mouse and human. also, our lysates are all from untransfected cells. Best regards,

Read More

Question
Answer

No. We do not plan to make antibodies to the pro-domain of BACE, however, we offer custom antibody production at greatly reduced prices.

Read More

11-15 of 15 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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