Question (50707) | Anti-BACE1 antibody (ab2077)

Go to datasheet (ab2077)

Question

Not seeing the correct size band in wetern blotiing

Answer

Thank you for calling Abcam yesterday.

I am sorry about the issues you have been having with ab2077 in western blot. Having talked to my colleagues, I think the best way to move forward with this is to either replace the antibody that you currently have or to refund the cost of the original purchase.

I do not think that there are any protocol tips that I could give that would help resolve the banding pattern that you are seeing and can only assume that the vial of ab2077 that you received is not working correctly.

A few alternative BACE1 antibodies to consider are:

https://www.abcam.com/BACE1-antibody-EPR3956-ab108394.html
https://www.abcam.com/BACE1-antibody-ab63954.html



If either of these antibodies would work for you (or if there is another BACE1 antibody that you would like to try), please let me know and I will be happy to send it out to you.

I look forward to your reply.

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