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Not seeing the correct size band in wetern blotiing
Asked on Jun 13 2012
Thank you for calling Abcam yesterday.
I am sorry about the issues you have been having with ab2077 in western blot. Having talked to my colleagues, I think the best way to move forward with this is to either replace the antibody that you currently have or to refund the cost of the original purchase.
I do not think that there are any protocol tips that I could give that would help resolve the banding pattern that you are seeing and can only assume that the vial of ab2077 that you received is not working correctly.
A few alternative BACE1 antibodies to consider are:
If either of these antibodies would work for you (or if there is another BACE1 antibody that you would like to try), please let me know and I will be happy to send it out to you.
I look forward to your reply.
Answered on Jun 13 2012