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I understand that you can't send all the reagents, but I've been working on this project for too long to quit. I don't think anything's wrong with the ab2077 antibody. Would you be able to send me only the 3T3 lysate? I need 30 ul to run at least 2 lanes. If that works, then I'll order (and pay for) more ab2077 to run the experiment to completion.
Asked on Jun 18 2012
Thank you for your reply.
If sending you a new aliquot of the cell lysate would help you to complete your experiments, then I would be happy to do so. However, I am confused as to why you think that the current vial of ab2077 that you have is ok when it is not giving the expected signal. I was hoping that if I send you a different antibody against the same target, then you would be able to detect your band of interest.
As I mentioned previously, I would be happy to send a new vial of cell lysate, I just wanted to confirm with you the course of action that you wish to take and to double check that you did not want to try a different primary antibody.
I look forward to your reply.
Answered on Jun 19 2012