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The ab2077 I have was giving the expected signal for my past several lysates. To verify the exact band, since I was getting one at approx 70 and another at approx 55/60, I used the mouse lysate. The strong band at 100 kD for the mouse lysate was unexpected and confusing, leading me to believe the mouse lysate wasn't good. I thought running it again with the mouse lysate in the middle of the gel might clarify where the positive control was appearing. I also thought that even if I ran this again with a new vial of ab2077, I still wouldn't have a positive control to verify my band (and appease future reviewers).
Having said that, perhaps you're right that I should at least try another ab2077. I'd rather do that then try a different primary antibody. So, go ahead and send a new ab2077. If I get the same thing, then I'll have to decide how to proceed to verify that the bands I'm seeing are indeed the glycosylated and non-glycosylated forms for BACE1.
Asked on Jun 19 2012
Thank you for your reply.
I am sending you a new vial of ab2077, your new order number is ** and you should have the new antibody by 10.30am tomorrow morning. If there is anything else I can help you with, or if you have any issues with the new vial of the antibody please let me know.
I hope your experiments go well.
Answered on Jun 19 2012