Question (66155) | Anti-BACE1 antibody (ab2077)

Go to datasheet (ab2077)

Question

Product code: 2077
Lot number: GR1723-1
Inquiry: Hi Technical, We have a customer who is experiencing problems with this antibody. Please find attached the customer's questionnaire.

Answer

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from our Anti-BACE1 antibody (ab2077) .

The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.

I would like to reassure you that ab2077 is tested and covered by our 6 month guarantee for use in WB and mouse as well as human samples. In the event that a product is not functioning in the applications cited on the product data sheet, we will be pleased to provide a credit note or free of charge replacement.

Regrettably reviewing all the details there are not many suggestions we can make to improve the protocol. However, I would like to investigate the case a bit further for you. Before deciding how to proceed with this particular case, I would appreciate if you can confirm some details of the procedure.

1. Could you please confirm if a loading control was included and what the results were? A loading control assures that the samples used are of good quality.

2. We have some data that suggest that the BACE1 protein is difficult to transfer to the membrane in WB application. I would appreciate if you could confirm if the transfer of protein onto the membrane was assased and what was the result?

3. Could you confirm the isotype that is recognised by the secondary antibody and weather it is known to work well with other primary antibodies?

Thank you very much for your cooperation. Please do not hesitate to contact me again with the further requested details. I look forward to your answer and am sure we will be able to find a solution.

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