Immunocytochemistry/ Immunofluorescence abreview for Anti-BACE1 antibody

Average
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (human glioblastoma cell line D54MG)
Specification
human glioblastoma cell line D54MG
Fixative
Paraformaldehyde
Permeabilization
Yes - 0,1% Triton X 100
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C

Other product details

Dilution
1/50
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.5% BSA in PBS

Secondary antibody

Name
Non-Abcam antibody was used: anti-rabbit TRITC conjugated T6778
Host species: Goat
Clonality: Polyclonal
Conjugation: TRITC
Dilution
1/400

Additional data

Additional Notes
we found only slight staining in the membrane but good staining near the nucleus. Nucleii are counterstained with DAPI.

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Submitted Aug 30 2011

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