Overview

  • Product name

  • Description

    Rabbit polyclonal to BACE1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Pig, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human BACE1 (internal sequence). The exact sequence is proprietary.
    Database link: P56817

  • Positive control

    • WB: HeLa whole cell lysate; human brain tissue extract. IHC-P: Mouse lung tissue; U87 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab228843 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 56 kDa.
IHC-P 1/100 - 1/1000.

Target

  • Function

    Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase.
  • Tissue specificity

    Expressed at high levels in the brain and pancreas. In the brain, expression is highest in the substantia nigra, locus coruleus and medulla oblongata.
  • Sequence similarities

    Belongs to the peptidase A1 family.
  • Domain

    The transmembrane domain is necessary for its activity. It determines its late Golgi localization and access to its substrate, APP.
  • Post-translational
    modifications

    Glycosylated.
  • Cellular localization

    Membrane. Golgi apparatus > trans-Golgi network. Endoplasmic reticulum. Endosome. Cell surface. Predominantly localized to the later Golgi/trans-Golgi network (TGN) and minimally detectable in the early Golgi compartments. A small portion is also found in the endoplasmic reticulum, endosomes and on the cell surface.
  • Information by UniProt
  • Database links

  • Alternative names

    • APP beta secretase antibody
    • Asp 2 antibody
    • ASP2 antibody
    • Aspartyl protease 2 antibody
    • BACE 1 antibody
    • BACE antibody
    • BACE1 antibody
    • BACE1_HUMAN antibody
    • Beta secretase 1 antibody
    • Beta secretase antibody
    • Beta site amyloid beta A4 precursor protein cleaving enzyme antibody
    • Beta site amyloid precursor protein cleaving enzyme 1 antibody
    • Beta site amyloid precursor protein cleaving enzyme antibody
    • Beta site APP cleaving enzyme 1 antibody
    • Beta site APP cleaving enzyme antibody
    • Beta-secretase 1 antibody
    • Beta-site amyloid precursor protein cleaving enzyme 1 antibody
    • Beta-site APP cleaving enzyme 1 antibody
    • FLJ90568 antibody
    • HSPC104 antibody
    • Memapsin 2 antibody
    • Memapsin-2 antibody
    • Memapsin2 antibody
    • Membrane associated aspartic protease 2 antibody
    • Membrane-associated aspartic protease 2 antibody
    • Transmembrane aspartic proteinase Asp2 antibody
    see all

Images

  • Paraffin-embedded mouse lung tissue stained for BACE1 with BACE1 at 1/500 dilution in immunohistochemical analysis.

  • Anti-BACE1 antibody (ab228843) at 1/2000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

    Developed using the ECL technique.

    Predicted band size: 56 kDa



    7.5% SDS-PAGE

  • Paraffin-embedded U87 (human) xenograft stained for BACE1 with BACE1 at 1/500 dilution in immunohistochemical analysis.

  • Anti-BACE1 antibody (ab228843) at 1/1000 dilution + Human brain tissue extracts at 30 µg

    Developed using the ECL technique.

    Predicted band size: 56 kDa



    7.5% SDS-PAGE

References

ab228843 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab228843.
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