Product nameAnti-BACE1 antibody
See all BACE1 primary antibodies
DescriptionRabbit polyclonal to BACE1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Macaque monkey
- This antibody gave a positive signal in the following lysates: Rat Lung Tissue HepG2 Whole Cell Mouse Kidney Tissue Human Liver Tissue (Data not shown)
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab63954 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 56, 70 kDa (predicted molecular weight: 56 kDa).|
FunctionResponsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase.
Tissue specificityExpressed at high levels in the brain and pancreas. In the brain, expression is highest in the substantia nigra, locus coruleus and medulla oblongata.
Sequence similaritiesBelongs to the peptidase A1 family.
DomainThe transmembrane domain is necessary for its activity. It determines its late Golgi localization and access to its substrate, APP.
Cellular localizationMembrane. Golgi apparatus > trans-Golgi network. Endoplasmic reticulum. Endosome. Cell surface. Predominantly localized to the later Golgi/trans-Golgi network (TGN) and minimally detectable in the early Golgi compartments. A small portion is also found in the endoplasmic reticulum, endosomes and on the cell surface.
- Information by UniProt
- APP beta secretase antibody
- Asp 2 antibody
- ASP2 antibody
All lanes : Anti-BACE1 antibody (ab63954) at 1 µg/ml
Lane 1 : Lung (Rat) Tissue Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : Kidney (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 56 kDa
Observed band size: 56,70 kDa why is the actual band size different from the predicted?
Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands.
BACE1 is known to run at 70 kDa due to potential glycosolation sites. It is beleived the 56 kDa band may correspond to the unglycosylated form of the protein.
ab63954 has not yet been referenced specifically in any publications.