Anti-BACE1 antibody [EPR19523] (ab183612)
- Datasheet
- References (4)
- Protocols
Overview
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Product nameAnti-BACE1 antibody [EPR19523]
See all BACE1 primary antibodies -
DescriptionRabbit monoclonal [EPR19523] to BACE1
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Host speciesRabbit
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Tested applicationsSuitable for: IHC-P, WB, IP, IHC-Frmore details
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Species reactivityReacts with: Mouse, Rat
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Immunogen
Recombinant fragment within Mouse BACE1 aa 50-350. The exact sequence is proprietary.
Database link: P56818 -
Positive control
- WB: Mouse and rat hippocampus and brain lysates. IHC-P: Mouse hippocampus and cerebrum tissues; rat cerebrum tissue. IHC-Fr: Mouse hippocampus tissue. IP: Rat and mouse hippocampus whole cell lysates.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberEPR19523
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab183612 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. IHC-P is recommended for mouse only. Binding in rat is weak under our experimental conditions and requires further optimization. |
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| WB | 1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 56 kDa). | |
| IP | 1/50. | |
| IHC-Fr | 1/250. IHC-Fr is recommended for mouse only. |
Target
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FunctionResponsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase.
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Tissue specificityExpressed at high levels in the brain and pancreas. In the brain, expression is highest in the substantia nigra, locus coruleus and medulla oblongata.
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Sequence similaritiesBelongs to the peptidase A1 family.
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DomainThe transmembrane domain is necessary for its activity. It determines its late Golgi localization and access to its substrate, APP.
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Post-translational
modificationsGlycosylated. -
Cellular localizationMembrane. Golgi apparatus > trans-Golgi network. Endoplasmic reticulum. Endosome. Cell surface. Predominantly localized to the later Golgi/trans-Golgi network (TGN) and minimally detectable in the early Golgi compartments. A small portion is also found in the endoplasmic reticulum, endosomes and on the cell surface.
- Information by UniProt
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Database links
- Entrez Gene: 23821 Mouse
- Entrez Gene: 29392 Rat
- SwissProt: P56818 Mouse
- SwissProt: P56819 Rat
- Unigene: 24044 Mouse
- Unigene: 207201 Rat
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Alternative names
- APP beta secretase antibody
- Asp 2 antibody
- ASP2 antibody
see all
Images
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![Western blot - Anti-BACE1 antibody [EPR19523] (ab183612)](https://www.abcam.com/ps/products/183/ab183612/Images/ab183612-299642-ab182KOWBJRIZ.jpg)
Western blot - Anti-BACE1 antibody [EPR19523] (ab183612)Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: BACE1 knockout HAP1 whole cell lysate (20 µg)
Lane 3: SHSY5Y whole cell lysate (20 µg)Lanes 1 - 3: Merged signal (red and green). Green - ab183612 observed at 68 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab183612 was shown to specifically react with BACE1 in wild-type HAP1 cells as signal was lost in BACE1 knockout cells. Wild-type and BACE1 knockout samples were subjected to SDS-PAGE. ab183612 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-BACE1 antibody [EPR19523] (ab183612)](https://www.abcam.com/ps/products/183/ab183612/Images/ab183612-256390-183612WBa.jpg)
Western blot - Anti-BACE1 antibody [EPR19523] (ab183612)All lanes : Anti-BACE1 antibody [EPR19523] (ab183612) at 1/1000 dilution
Lane 1 : Mouse hippocampus lysate
Lane 2 : Rat brain lysate
Lane 3 : Rat hippocampus lysate
Lane 4 : Mouse ovary lysate
Lane 5 : Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate
Lane 6 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 7 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 56 kDa
Observed band size: 68 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1 and 2: 1 minute; Lane 3,4,5,6,7 and 8: 3 minutes.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACE1 antibody [EPR19523] (ab183612)](https://www.abcam.com/ps/products/183/ab183612/Images/ab183612-256383-183612IHCf.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACE1 antibody [EPR19523] (ab183612)Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling BACE1 with ab183612 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on some neurons of the rat cerebrum is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Binding in rat was weak under our experimental conditions and requires further optimization.
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![Western blot - Anti-BACE1 antibody [EPR19523] (ab183612)](https://www.abcam.com/ps/products/183/ab183612/Images/ab183612-256389-wb-2.jpg)
Western blot - Anti-BACE1 antibody [EPR19523] (ab183612)Anti-BACE1 antibody [EPR19523] (ab183612) at 1/1000 dilution + Mouse brain lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 56 kDa
Observed band size: 68 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsAn additional band was observed at 70 kD. The expression profile is consistent with what has been described in the literature (PMID: 22741101).
Blocking/Dilution buffer: 5% NFDM/TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACE1 antibody [EPR19523] (ab183612)](https://www.abcam.com/ps/products/183/ab183612/Images/ab183612-256387-183612IHCa.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACE1 antibody [EPR19523] (ab183612)Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling BACE1 with ab183612 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on mouse Hilar region of the dentate gyrus is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACE1 antibody [EPR19523] (ab183612)](https://www.abcam.com/ps/products/183/ab183612/Images/ab183612-256386-183612IHCb.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACE1 antibody [EPR19523] (ab183612)Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling BACE1 with ab183612 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on neurons of the mouse cerebrum is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACE1 antibody [EPR19523] (ab183612)](https://www.abcam.com/ps/products/183/ab183612/Images/ab183612-256388-183612IHCc.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACE1 antibody [EPR19523] (ab183612)Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling BACE1 with ab183612 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on mouse liver. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACE1 antibody [EPR19523] (ab183612)](https://www.abcam.com/ps/products/183/ab183612/Images/ab183612-259003-183612-ihc.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACE1 antibody [EPR19523] (ab183612)Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue labelling BACE1 with ab183612 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. The sample was counterstained with hematoxylin. Antigen retrieval was performed using Tris/EDTA buffer; pH 9.0.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Binding in rat was weak under our experimental conditions and requires further optimization.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACE1 antibody [EPR19523] (ab183612)](https://www.abcam.com/ps/products/183/ab183612/Images/ab183612-259007-183612-ihc2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACE1 antibody [EPR19523] (ab183612)Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labelling BACE1 with ab183612 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. The sample was counterstained with hematoxylin. Antigen retrieval was performed using Tris/EDTA buffer; pH 9.0.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Binding in rat was weak under our experimental conditions and requires further optimization.
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![Immunohistochemistry (Frozen sections) - Anti-BACE1 antibody [EPR19523] (ab183612)](https://www.abcam.com/ps/products/183/ab183612/Images/ab183612-256382-183612IHCIF.jpg)
Immunohistochemistry (Frozen sections) - Anti-BACE1 antibody [EPR19523] (ab183612)Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse hippocampus tissue labeling BACE1 with ab183612 at 1/250 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). The result showed mainly cytoplasmic staining on mouse hippocampus. The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
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![Immunoprecipitation - Anti-BACE1 antibody [EPR19523] (ab183612)](https://www.abcam.com/ps/products/183/ab183612/Images/ab183612-256381-ip-1.jpg)
Immunoprecipitation - Anti-BACE1 antibody [EPR19523] (ab183612)BACE1 was immunoprecipitated from 1mg of rat hippocampus whole cell lysate with ab183612 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab183612 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366), was used as secondary antibody at 1/10000 dilution.
Lane 1: Rat hippocampus whole cell lysate, 10µg (Input).
Lane 2: ab183612 IP in Rat hippocampus whole cell lysate.
Lane 3: Rabbit IgG,monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab183612 in rat hippocampus whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5 seconds.
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![Immunoprecipitation - Anti-BACE1 antibody [EPR19523] (ab183612)](https://www.abcam.com/ps/products/183/ab183612/Images/ab183612-256380-ip-2.jpg)
Immunoprecipitation - Anti-BACE1 antibody [EPR19523] (ab183612)BACE1 was immunoprecipitated from 1mg of mouse hippocampus whole cell lysate with ab183612 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab183612 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366), was used as secondary antibody at 1/10000 dilution.
Lane 1: Mouse hippocampus whole cell lysate, 10µg (Input).
Lane 2: ab183612 IP in mouse hippocampus whole cell lysate.
Lane 3: Rabbit IgG,monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab183612 in Mouse hippocampus whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
Datasheets and documents
References
This product has been referenced in:
- Huang Y et al. Chinese Herbal Medicine Xueshuantong Enhances Cerebral Blood Flow and Improves Neural Functions in Alzheimer's Disease Mice. J Alzheimers Dis 63:1089-1107 (2018). Read more (PubMed: 29710701) »
- Meng G et al. Application of Ferulic Acid for Alzheimer's Disease: Combination of Text Mining and Experimental Validation. Front Neuroinform 12:31 (2018). Read more (PubMed: 29896095) »
