Recombinant Anti-BACE1 antibody [EPR3956] (ab108394)
![Western blot - Anti-BACE1 antibody [EPR3956] (ab108394)](https://www.abcam.com/ps/products/108/ab108394/Images/ab108394-288640-anti-bace1-antibody-epr3956-western-blot.jpg "Western blot - Anti-BACE1 antibody [EPR3956] (ab108394)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3956] to BACE1
- Suitable for: WB, IP
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-BACE1 antibody [EPR3956]
See all BACE1 primary antibodies -
Description
Rabbit monoclonal [EPR3956] to BACE1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: SH-SY5Y cell lysate and human fetal, mouse and rat brain tissue lysates. IP: Human fetal brain tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3956 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab108394 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (2) |
1/1000 - 1/10000. Predicted molecular weight: 56 kDa.
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| IP |
1/10 - 1/100.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 56 kDa. |
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IP
1/10 - 1/100. |
Target
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Function
Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. -
Tissue specificity
Expressed at high levels in the brain and pancreas. In the brain, expression is highest in the substantia nigra, locus coruleus and medulla oblongata. -
Sequence similarities
Belongs to the peptidase A1 family. -
Domain
The transmembrane domain is necessary for its activity. It determines its late Golgi localization and access to its substrate, APP. -
Post-translational
modificationsGlycosylated. -
Cellular localization
Membrane. Golgi apparatus > trans-Golgi network. Endoplasmic reticulum. Endosome. Cell surface. Predominantly localized to the later Golgi/trans-Golgi network (TGN) and minimally detectable in the early Golgi compartments. A small portion is also found in the endoplasmic reticulum, endosomes and on the cell surface. - Information by UniProt
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Database links
- Entrez Gene: 23621 Human
- Entrez Gene: 23821 Mouse
- Entrez Gene: 29392 Rat
- Omim: 604252 Human
- SwissProt: P56817 Human
- SwissProt: P56818 Mouse
- SwissProt: P56819 Rat
- Unigene: 504003 Human
see all -
Alternative names
- APP beta secretase antibody
- Asp 2 antibody
- ASP2 antibody
see all
Images
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Western blot - Anti-BACE1 antibody [EPR3956] (ab108394)
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: BACE1 knockout HAP1 whole cell lysate (20 µg)
Lane 3: SHSY5Y whole cell lysate (20 µg)
Lane 4: Human brain whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab108394 observed at 70 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab108394 was shown to recognize BACE1 in wild type cells as signal was lost at the expected MW in BACE1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and BACE1 knockout samples were subjected to SDS-PAGE. Ab108394 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-BACE1 antibody [EPR3956] (ab108394)](https://www.abcam.com/ps/products/108/ab108394/Images/ab108394-253077-anti-bace1-antibody-epr3956-western-blot.jpg)
Western blot - Anti-BACE1 antibody [EPR3956] (ab108394)All lanes : Anti-BACE1 antibody [EPR3956] (ab108394) at 1/10000 dilution (purified)
Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution
Predicted band size: 56 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-BACE1 antibody [EPR3956] (ab108394)](https://www.abcam.com/ps/products/108/ab108394/Images/ab108394-253076-anti-bace1-antibody-epr3956-western-blot.jpg)
Western blot - Anti-BACE1 antibody [EPR3956] (ab108394)Anti-BACE1 antibody [EPR3956] (ab108394) at 1/10000 dilution (purified) + Human fetal brain tissue lysate at 20 µg
Secondary
Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution
Predicted band size: 56 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
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![Immunoprecipitation - Anti-BACE1 antibody [EPR3956] (ab108394)](https://www.abcam.com/ps/products/108/ab108394/Images/ab108394-252965-anti-bace1-antibody-epr3956-immunoprecipitation.jpg)
Immunoprecipitation - Anti-BACE1 antibody [EPR3956] (ab108394)ab108394 (purified) at 1/40 immunoprecipitating BACE1 in human fetal brain whole tissue lysate.
Lane 1 (input): human fetal brain whole tissue lysate (10µg)
Lane 2 (+): ab108394 + human fetal brain whole tissue lysate (10µg).
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab108394 in human fetal brain whole tissue lysate.
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1500 dilution.
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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![Western blot - Anti-BACE1 antibody [EPR3956] (ab108394)](https://www.abcam.com/ps/products/108/ab108394/Images/ab108394-121055-anti-bace1-antibody-epr3956-western-blot.jpg)
Western blot - Anti-BACE1 antibody [EPR3956] (ab108394)All lanes : Anti-BACE1 antibody [EPR3956] (ab108394) at 1/1000 dilution (unpurified)
Lane 1 : Fetal brain lysate
Lane 2 : SH-SY5Y lysate
Lane 3 : Mouse brain lysate
Lane 4 : Rat brain lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 56 kDa -
![Anti-BACE1 antibody [EPR3956] (ab108394)](https://www.abcam.com/ps/products/108/ab108394/Images/ab108394-6-benefits-of-recombinant-antibodies.png)
Anti-BACE1 antibody [EPR3956] (ab108394)
Protocols
Datasheets and documents
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Datasheet download
References (44)
ab108394 has been referenced in 44 publications.
- Pi T et al. High Methionine Diet-Induced Alzheimer's Disease like Symptoms Are Accompanied by 5-Methylcytosine Elevated Levels in the Brain. Behav Neurol 2021:6683318 (2021). PubMed: 33880134
- Liu Y et al. Plasmalogen attenuates the development of hepatic steatosis and cognitive deficit through mechanism involving p75NTR inhibition. Redox Biol 43:102002 (2021). PubMed: 33984602
- Langness VF et al. Cholesterol-lowering drugs reduce APP processing to Aß by inducing APP dimerization. Mol Biol Cell 32:247-259 (2021). PubMed: 33296223
- Maitra S et al. Phenanthroline impairs ßAPP processing and expression, increases p53 protein levels and induces cell cycle arrest in human neuroblastoma cells. Brain Res Bull 170:29-38 (2021). PubMed: 33556560
- Zhong M et al. Nasal Delivery of D-Penicillamine Hydrogel Upregulates a Disintegrin and Metalloprotease 10 Expression via Melatonin Receptor 1 in Alzheimer's Disease Models. Front Aging Neurosci 13:660249 (2021). PubMed: 33935689
