Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-BACE1 antibody [EPR3956] (ab108394)

Reviews (2)Q&A (2)References (22)

Overview

  • Product name

    Anti-BACE1 antibody [EPR3956]
    See all BACE1 primary antibodies

  • Description

    Rabbit monoclonal [EPR3956] to BACE1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human BACE1. The exact sequence is proprietary.

  • Positive control

    • WB: SH-SY5Y cell lysate and human fetal, mouse and rat brain tissue lysates. IP: Human fetal brain tissue lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab108394 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 56 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.

    • Target

    • Function

      Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase.

    • Tissue specificity

      Expressed at high levels in the brain and pancreas. In the brain, expression is highest in the substantia nigra, locus coruleus and medulla oblongata.

    • Sequence similarities

      Belongs to the peptidase A1 family.

    • Domain

      The transmembrane domain is necessary for its activity. It determines its late Golgi localization and access to its substrate, APP.

    • Post-translational
      modifications

      Glycosylated.

    • Cellular localization

      Membrane. Golgi apparatus > trans-Golgi network. Endoplasmic reticulum. Endosome. Cell surface. Predominantly localized to the later Golgi/trans-Golgi network (TGN) and minimally detectable in the early Golgi compartments. A small portion is also found in the endoplasmic reticulum, endosomes and on the cell surface.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • APP beta secretase antibody
      • Asp 2 antibody
      • ASP2 antibody
      • Aspartyl protease 2 antibody
      • BACE 1 antibody
      • BACE antibody
      • BACE1 antibody
      • BACE1_HUMAN antibody
      • Beta secretase 1 antibody
      • Beta secretase antibody
      • Beta site amyloid beta A4 precursor protein cleaving enzyme antibody
      • Beta site amyloid precursor protein cleaving enzyme 1 antibody
      • Beta site amyloid precursor protein cleaving enzyme antibody
      • Beta site APP cleaving enzyme 1 antibody
      • Beta site APP cleaving enzyme antibody
      • Beta-secretase 1 antibody
      • Beta-site amyloid precursor protein cleaving enzyme 1 antibody
      • Beta-site APP cleaving enzyme 1 antibody
      • FLJ90568 antibody
      • HSPC104 antibody
      • Memapsin 2 antibody
      • Memapsin-2 antibody
      • Memapsin2 antibody
      • Membrane associated aspartic protease 2 antibody
      • Membrane-associated aspartic protease 2 antibody
      • Transmembrane aspartic proteinase Asp2 antibody
      see all

    Images

    • Western blot - Anti-BACE1 antibody [EPR3956] (ab108394)
      Western blot - Anti-BACE1 antibody [EPR3956] (ab108394)

      Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: BACE1 knockout HAP1 whole cell lysate (20 µg)
      Lane 3: SHSY5Y whole cell lysate (20 µg)
      Lane 4: Human brain whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab108394 observed at 70 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab108394 was shown to recognize BACE1 in wild type cells as signal was lost at the expected MW in BACE1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and BACE1 knockout samples were subjected to SDS-PAGE. Ab108394 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-BACE1 antibody [EPR3956] (ab108394)
      Western blot - Anti-BACE1 antibody [EPR3956] (ab108394)
      All lanes : Anti-BACE1 antibody [EPR3956] (ab108394) at 1/10000 dilution (purified)

      Lane 1 : Mouse brain tissue lysate
      Lane 2 : Rat brain tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

      Predicted band size: 56 kDa
      Observed band size: 70 kDa
      why is the actual band size different from the predicted?



      Blocking and dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-BACE1 antibody [EPR3956] (ab108394)
      Western blot - Anti-BACE1 antibody [EPR3956] (ab108394)
      Anti-BACE1 antibody [EPR3956] (ab108394) at 1/10000 dilution (purified) + Human fetal brain tissue lysate at 20 µg

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

      Predicted band size: 56 kDa
      Observed band size: 70 kDa why is the actual band size different from the predicted?



      Blocking and dilution buffer: 5% NFDM/TBST.

    • Immunoprecipitation - Anti-BACE1 antibody [EPR3956] (ab108394)
      Immunoprecipitation - Anti-BACE1 antibody [EPR3956] (ab108394)

      ab108394 (purified) at 1/40 immunoprecipitating BACE1 in human fetal brain whole tissue lysate.

      Lane 1 (input): human fetal brain whole tissue lysate (10µg)

      Lane 2 (+): ab108394 + human fetal brain whole tissue lysate (10µg).

      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab108394 in human fetal brain whole tissue lysate.

      For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1500 dilution.

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Western blot - Anti-BACE1 antibody [EPR3956] (ab108394)
      Western blot - Anti-BACE1 antibody [EPR3956] (ab108394)
      All lanes : Anti-BACE1 antibody [EPR3956] (ab108394) at 1/1000 dilution (unpurified)

      Lane 1 : Fetal brain lysate
      Lane 2 : SH-SY5Y lysate
      Lane 3 : Mouse brain lysate
      Lane 4 : Rat brain lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 56 kDa

    References

    Publishing research using ab108394? Please let us know so that we can cite the reference in this datasheet.

    ab108394 has been referenced in 22 publications.

    • Bourassa P  et al. Beta-amyloid pathology in human brain microvessel extracts from the parietal cortex: relation with cerebral amyloid angiopathy and Alzheimer's disease. Acta Neuropathol 137:801-823 (2019). PubMed: 30729296
    • Shukla M  et al. Methamphetamine regulates ßAPP processing in human neuroblastoma cells. Neurosci Lett 701:20-25 (2019). PubMed: 30771376
    • Al-Atrache Z  et al. Astrocytes infected with Chlamydia pneumoniae demonstrate altered expression and activity of secretases involved in the generation of ß-amyloid found in Alzheimer disease. BMC Neurosci 20:6 (2019). PubMed: 30786875
    • Haroon MM  et al. Delivery of BACE1 siRNA mediated by TARBP-BTP fusion protein reduces ß-amyloid deposits in a transgenic mouse model of Alzheimer's disease. J Biosci 44:N/A (2019). PubMed: 30837353
    • Hartmann S  et al. ß-Secretase BACE1 Promotes Surface Expression and Function of Kv3.4 at Hippocampal Mossy Fiber Synapses. J Neurosci 38:3480-3494 (2018). PubMed: 29507146
    View all Publications for this product

    Customer reviews and Q&As

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    1-2 of 2 Abreviews

    Western blot abreview for Anti-BACE1 antibody [EPR3956]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Hippocampus)
    Gel Running Conditions
    Non-reduced Denaturing
    Loading amount
    40 µg
    Specification
    Hippocampus
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Dr. Hyeon Joo Ham

    Verified customer

    Submitted Dec 28 2018

    Western blot abreview for Anti-BACE1 antibody [EPR3956]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Brain)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native) (8%)
    Loading amount
    30 µg
    Treatment
    250 μg/kg LPS for 1 week
    Specification
    Brain
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Ms. Ji Hye Han

    Verified customer

    Submitted Jul 05 2018

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