Overview

  • Product name
    Anti-BACE1 antibody [EPR3956] - BSA and Azide free
    See all BACE1 primary antibodies
  • Description
    Rabbit monoclonal [EPR3956] to BACE1 - BSA and Azide free
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human BACE1. The exact sequence is proprietary.

  • Positive control
    • WB: Wild type HAP1 whole cell lysate; SH-SY5Y whole cell lysate; Human brain whole cell lysate.
  • General notes

    The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®).

    ab237595 is a PBS-only buffer format of ab108394. Please refer to ab108394 for recommended dilutions, protocols, and image data.

     

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab237595 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 56 kDa.
IP Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function
      Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase.
    • Tissue specificity
      Expressed at high levels in the brain and pancreas. In the brain, expression is highest in the substantia nigra, locus coruleus and medulla oblongata.
    • Sequence similarities
      Belongs to the peptidase A1 family.
    • Domain
      The transmembrane domain is necessary for its activity. It determines its late Golgi localization and access to its substrate, APP.
    • Post-translational
      modifications
      Glycosylated.
    • Cellular localization
      Membrane. Golgi apparatus > trans-Golgi network. Endoplasmic reticulum. Endosome. Cell surface. Predominantly localized to the later Golgi/trans-Golgi network (TGN) and minimally detectable in the early Golgi compartments. A small portion is also found in the endoplasmic reticulum, endosomes and on the cell surface.
    • Information by UniProt
    • Database links
    • Alternative names
      • APP beta secretase antibody
      • Asp 2 antibody
      • ASP2 antibody
      • Aspartyl protease 2 antibody
      • BACE 1 antibody
      • BACE antibody
      • BACE1 antibody
      • BACE1_HUMAN antibody
      • Beta secretase 1 antibody
      • Beta secretase antibody
      • Beta site amyloid beta A4 precursor protein cleaving enzyme antibody
      • Beta site amyloid precursor protein cleaving enzyme 1 antibody
      • Beta site amyloid precursor protein cleaving enzyme antibody
      • Beta site APP cleaving enzyme 1 antibody
      • Beta site APP cleaving enzyme antibody
      • Beta-secretase 1 antibody
      • Beta-site amyloid precursor protein cleaving enzyme 1 antibody
      • Beta-site APP cleaving enzyme 1 antibody
      • FLJ90568 antibody
      • HSPC104 antibody
      • Memapsin 2 antibody
      • Memapsin-2 antibody
      • Memapsin2 antibody
      • Membrane associated aspartic protease 2 antibody
      • Membrane-associated aspartic protease 2 antibody
      • Transmembrane aspartic proteinase Asp2 antibody
      see all

    Images

    • ab108394 (purified) at 1/40 immunoprecipitating BACE1 in human fetal brain whole tissue lysate.

      Lane 1 (input): human fetal brain whole tissue lysate (10µg)

      Lane 2 (+): ab108394 + human fetal brain whole tissue lysate (10µg).

      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab108394 in human fetal brain whole tissue lysate.

      For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1500 dilution.

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108394).

    • Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: BACE1 knockout HAP1 whole cell lysate (20 µg)
      Lane 3: SH-SY5Y whole cell lysate (20 µg)
      Lane 4: Human brain whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab108394 observed at 70 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab108394 was shown to recognize BACE1 in wild type cells as signal was lost at the expected MW in BACE1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and BACE1 knockout samples were subjected to SDS-PAGE. Ab108394 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108394).

    References

    ab237595 has not yet been referenced specifically in any publications.

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