• Product name

  • Description

    Rabbit polyclonal to BACE2
  • Host species

  • Tested applications

    Suitable for: WB, ICC, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human BACE2 aa 441-457.


    (Peptide available as ab5869)

  • Positive control

    • WB: SY-SY5Y cells overexpressing the human gene.



Our Abpromise guarantee covers the use of ab5670 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml.
ICC 1/10 - 1/200.
ICC/IF 1/10 - 1/200.
IHC-P Use at an assay dependent concentration.


  • Function

    Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves APP, between residues 690 and 691, leading to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. It has also been shown that it can cleave APP between residues 671 and 672.
  • Tissue specificity

    Brain. Present in neurons within the hippocampus, frontal cortex and temporal cortex (at protein level). Expressed at low levels in most peripheral tissues and at higher levels in colon, kidney, pancreas, placenta, prostate, stomach and trachea. Expressed at low levels in the brain. Found in spinal cord, medulla oblongata, substantia nigra and locus coruleus. Expressed in the ductal epithelium of both normal and malignant prostate.
  • Sequence similarities

    Belongs to the peptidase A1 family.
  • Post-translational

    Undergoes autoproteolytic cleavage.
  • Cellular localization

    Membrane. Golgi apparatus. Endoplasmic reticulum. Endosome. Cell surface.
  • Information by UniProt
  • Database links

  • Alternative names

    • ALP 56 antibody
    • ALP56 antibody
    • Asp 1 antibody
    • ASP1 antibody
    • ASP21 antibody
    • Aspartic Like Protease 56kDa antibody
    • Aspartic-like protease 56 kDa antibody
    • Aspartyl protease 1 antibody
    • BACE 2 antibody
    • Bace2 antibody
    • BACE2_HUMAN antibody
    • Beta Secretase 2 antibody
    • Beta Site Amyloid Beta A4 Precursor Protein Cleaving Enzyme 2 antibody
    • Beta-secretase 2 antibody
    • Beta-site amyloid precursor protein cleaving enzyme 2 antibody
    • Beta-site APP cleaving enzyme 2 antibody
    • Down region aspartic protease antibody
    • Downs Syndrome Region Aspartic Protease antibody
    • DRAP antibody
    • Memapsin 1 antibody
    • Memapsin-1 antibody
    • Membrane associated aspartic protease 1 antibody
    • Membrane-associated aspartic protease 1 antibody
    • Theta-secretase antibody
    see all


  • Immunocytochemistry/Immunofluorescence analysis of BACE2 (green) showing staining in the nucleus and cytoplasm of A549 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab5670 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

  • All lanes : Anti-BACE2 antibody (ab5670) at 1/500 dilution

    Lane 1 : Mouse spleen cell lysate
    Lane 2 : SH-SY5Y cell lysate
    Lane 3 : HL60 cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 25 µg per lane.


This product has been referenced in:

  • Cao K  et al. Exposure to fluoride aggravates the impairment in learning and memory and neuropathological lesions in mice carrying the APP/PS1 double-transgenic mutation. Alzheimers Res Ther 11:35 (2019). Read more (PubMed: 31010414) »
  • Holler CJ  et al. BACE2 expression increases in human neurodegenerative disease. Am J Pathol 180:337-50 (2012). Read more (PubMed: 22074738) »
See all 3 Publications for this product

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