Anti-BACE2 antibody (ab8024)
Key features and details
- Rabbit polyclonal to BACE2
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-BACE2 antibody
See all BACE2 primary antibodies -
Description
Rabbit polyclonal to BACE2 -
Host species
Rabbit -
Tested Applications & Species
Application Species IHC-P RatWB Human -
Immunogen
Synthetic peptide, corresponding to amino acids 496-511 of Human BACE 2.
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General notes
Beta-site APP Cleaving EnzymeThe Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab8024 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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IHC-P |
Rat
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WB |
Human
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Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 2 µg/ml.
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|
WB |
Use a concentration of 0.5 - 1 µg/ml.
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Notes |
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IHC-P
Use a concentration of 2 µg/ml. |
WB
Use a concentration of 0.5 - 1 µg/ml. |
Target
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Function
Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves APP, between residues 690 and 691, leading to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. It has also been shown that it can cleave APP between residues 671 and 672. -
Tissue specificity
Brain. Present in neurons within the hippocampus, frontal cortex and temporal cortex (at protein level). Expressed at low levels in most peripheral tissues and at higher levels in colon, kidney, pancreas, placenta, prostate, stomach and trachea. Expressed at low levels in the brain. Found in spinal cord, medulla oblongata, substantia nigra and locus coruleus. Expressed in the ductal epithelium of both normal and malignant prostate. -
Sequence similarities
Belongs to the peptidase A1 family. -
Post-translational
modificationsUndergoes autoproteolytic cleavage.
Glycosylated. -
Cellular localization
Membrane. Golgi apparatus. Endoplasmic reticulum. Endosome. Cell surface. - Information by UniProt
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Database links
- Entrez Gene: 25825 Human
- Entrez Gene: 288227 Rat
- Omim: 605668 Human
- SwissProt: Q9Y5Z0 Human
- SwissProt: Q6IE75 Rat
- Unigene: 529408 Human
- Unigene: 38581 Rat
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Alternative names
- ALP 56 antibody
- ALP56 antibody
- Asp 1 antibody
see all
Images
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Western Blot of human heart lysate labeling BACE2 with Anti-BACE2 antibody (ab8024) at 1µg/ml in (1) the absence and (2) the presence of blocking peptide..
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All lanes : Anti-BACE2 antibody (ab8024) at 1 µg/ml
Lane 1 : human heart tissue lysate with absence of blocking peptide
Lane 2 : human heart tissue lysate with presence of blocking peptide -
Immunohistochemistry of BACE2 in rat heart tissue with BACE2 antibody at 2 µg/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab8024 has not yet been referenced specifically in any publications.