Anti-BACE2 antibody (ab8024)
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Overview
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Product nameAnti-BACE2 antibody
See all BACE2 primary antibodies -
DescriptionRabbit polyclonal to BACE2
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide, corresponding to amino acids 496-511 of Human BACE 2.
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Positive control
- Human heart tissue lysate. This antibody gave a positive result when used in the following methanol fixed cell lines: MALME-3M
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General notesBeta-site APP Cleaving Enzyme
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C.
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Storage bufferPreservative: 0.02% Sodium azide
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Concentration information loading... -
PurityAffinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab8024 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. | |
| IHC-P | Use a concentration of 2 µg/ml. | |
| WB | Use a concentration of 0.5 - 1 µg/ml. Can be blocked with BACE2 peptide (ab8391). |
Target
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FunctionResponsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves APP, between residues 690 and 691, leading to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. It has also been shown that it can cleave APP between residues 671 and 672.
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Tissue specificityBrain. Present in neurons within the hippocampus, frontal cortex and temporal cortex (at protein level). Expressed at low levels in most peripheral tissues and at higher levels in colon, kidney, pancreas, placenta, prostate, stomach and trachea. Expressed at low levels in the brain. Found in spinal cord, medulla oblongata, substantia nigra and locus coruleus. Expressed in the ductal epithelium of both normal and malignant prostate.
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Sequence similaritiesBelongs to the peptidase A1 family.
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Post-translational
modificationsUndergoes autoproteolytic cleavage.
Glycosylated. -
Cellular localizationMembrane. Golgi apparatus. Endoplasmic reticulum. Endosome. Cell surface.
- Information by UniProt
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Database links
- Entrez Gene: 25825 Human
- Entrez Gene: 56175 Mouse
- Entrez Gene: 288227 Rat
- Omim: 605668 Human
- SwissProt: Q9Y5Z0 Human
- SwissProt: Q9JL18 Mouse
- SwissProt: Q6IE75 Rat
- Unigene: 529408 Human
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Alternative names
- ALP 56 antibody
- ALP56 antibody
- Asp 1 antibody
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Images
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Western blot - Anti-BACE2 antibody (ab8024)Western Blot of human heart lysate labeling BACE2 with Anti-BACE2 antibody (ab8024) at 1µg/ml in (1) the absence and (2) the presence of blocking peptide.. -
Immunocytochemistry/ Immunofluorescence - Anti-BACE2 antibody (ab8024)ICC/IF image of ab8024 stained MALME-3M cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab8024 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot - Anti-BACE2 antibody (ab8024)All lanes : Anti-BACE2 antibody (ab8024) at 1 µg/ml
Lane 1 : human heart tissue lysate with absence of blocking peptide
Lane 2 : human heart tissue lysate with presence of blocking peptide -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACE2 antibody (ab8024)Immunohistochemistry of BACE2 in rat heart tissue with BACE2 antibody at 2 µg/ml.
Datasheets and documents
References
ab8024 has not yet been referenced specifically in any publications.
