• Product name
  • Description
    Rabbit polyclonal to BACE2
  • Host species
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to amino acids 496-511 of Human BACE 2.

  • Positive control
    • Human heart tissue lysate. This antibody gave a positive result when used in the following methanol fixed cell lines: MALME-3M
  • General notes
    Beta-site APP Cleaving Enzyme



Our Abpromise guarantee covers the use of ab8024 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P Use a concentration of 2 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Can be blocked with BACE2 peptide (ab8391).


  • Function
    Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves APP, between residues 690 and 691, leading to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. It has also been shown that it can cleave APP between residues 671 and 672.
  • Tissue specificity
    Brain. Present in neurons within the hippocampus, frontal cortex and temporal cortex (at protein level). Expressed at low levels in most peripheral tissues and at higher levels in colon, kidney, pancreas, placenta, prostate, stomach and trachea. Expressed at low levels in the brain. Found in spinal cord, medulla oblongata, substantia nigra and locus coruleus. Expressed in the ductal epithelium of both normal and malignant prostate.
  • Sequence similarities
    Belongs to the peptidase A1 family.
  • Post-translational
    Undergoes autoproteolytic cleavage.
  • Cellular localization
    Membrane. Golgi apparatus. Endoplasmic reticulum. Endosome. Cell surface.
  • Information by UniProt
  • Database links
  • Alternative names
    • ALP 56 antibody
    • ALP56 antibody
    • Asp 1 antibody
    • ASP1 antibody
    • ASP21 antibody
    • Aspartic Like Protease 56kDa antibody
    • Aspartic-like protease 56 kDa antibody
    • Aspartyl protease 1 antibody
    • BACE 2 antibody
    • Bace2 antibody
    • BACE2_HUMAN antibody
    • Beta Secretase 2 antibody
    • Beta Site Amyloid Beta A4 Precursor Protein Cleaving Enzyme 2 antibody
    • Beta-secretase 2 antibody
    • Beta-site amyloid precursor protein cleaving enzyme 2 antibody
    • Beta-site APP cleaving enzyme 2 antibody
    • Down region aspartic protease antibody
    • Downs Syndrome Region Aspartic Protease antibody
    • DRAP antibody
    • Memapsin 1 antibody
    • Memapsin-1 antibody
    • Membrane associated aspartic protease 1 antibody
    • Membrane-associated aspartic protease 1 antibody
    • Theta-secretase antibody
    see all


  • Western Blot of human heart lysate labeling BACE2 with Anti-BACE2 antibody (ab8024) at 1µg/ml in (1) the absence and (2) the presence of blocking peptide..
  • ICC/IF image of ab8024 stained MALME-3M cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab8024 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-BACE2 antibody (ab8024) at 1 µg/ml

    Lane 1 : human heart tissue lysate with absence of blocking peptide
    Lane 2 : human heart tissue lysate with presence of blocking peptide
  • Immunohistochemistry of BACE2 in rat heart tissue with BACE2 antibody at 2 µg/ml.


ab8024 has not yet been referenced specifically in any publications.

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