Anti-BACE2 antibody (ab8024)

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Western blot - Anti-BACE2 antibody (ab8024)
  • Western blot - Anti-BACE2 antibody (ab8024)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACE2 antibody (ab8024)

Key features and details

  • Rabbit polyclonal to BACE2
  • Suitable for: IHC-P, WB
  • Reacts with: Rat, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-BACE2 antibody
    See all BACE2 primary antibodies

  • Description

    Rabbit polyclonal to BACE2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Rat
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide, corresponding to amino acids 496-511 of Human BACE 2.

  • General notes

    Beta-site APP Cleaving Enzyme

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab8024 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Rat
WB
Human
Application Abreviews Notes
IHC-P
Use a concentration of 2 µg/ml.
WB
Use a concentration of 0.5 - 1 µg/ml.
Notes
IHC-P
Use a concentration of 2 µg/ml.
WB
Use a concentration of 0.5 - 1 µg/ml.

Target

  • Function

    Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves APP, between residues 690 and 691, leading to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. It has also been shown that it can cleave APP between residues 671 and 672.

  • Tissue specificity

    Brain. Present in neurons within the hippocampus, frontal cortex and temporal cortex (at protein level). Expressed at low levels in most peripheral tissues and at higher levels in colon, kidney, pancreas, placenta, prostate, stomach and trachea. Expressed at low levels in the brain. Found in spinal cord, medulla oblongata, substantia nigra and locus coruleus. Expressed in the ductal epithelium of both normal and malignant prostate.

  • Sequence similarities

    Belongs to the peptidase A1 family.

  • Post-translational
    modifications

    Undergoes autoproteolytic cleavage.
    Glycosylated.

  • Cellular localization

    Membrane. Golgi apparatus. Endoplasmic reticulum. Endosome. Cell surface.
  • Information by UniProt

  • Database links

    • Alternative names

      • ALP 56 antibody
      • ALP56 antibody
      • Asp 1 antibody
      • ASP1 antibody
      • ASP21 antibody
      • Aspartic Like Protease 56kDa antibody
      • Aspartic-like protease 56 kDa antibody
      • Aspartyl protease 1 antibody
      • BACE 2 antibody
      • Bace2 antibody
      • BACE2_HUMAN antibody
      • Beta Secretase 2 antibody
      • Beta Site Amyloid Beta A4 Precursor Protein Cleaving Enzyme 2 antibody
      • Beta-secretase 2 antibody
      • Beta-site amyloid precursor protein cleaving enzyme 2 antibody
      • Beta-site APP cleaving enzyme 2 antibody
      • Down region aspartic protease antibody
      • Downs Syndrome Region Aspartic Protease antibody
      • DRAP antibody
      • Memapsin 1 antibody
      • Memapsin-1 antibody
      • Membrane associated aspartic protease 1 antibody
      • Membrane-associated aspartic protease 1 antibody
      • Theta-secretase antibody
      see all

    Images

    • Western blot - Anti-BACE2 antibody (ab8024)
      Western blot - Anti-BACE2 antibody (ab8024)
      Western Blot of human heart lysate labeling BACE2 with Anti-BACE2 antibody (ab8024) at 1µg/ml in (1) the absence and (2) the presence of blocking peptide..
    • Western blot - Anti-BACE2 antibody (ab8024)
      Western blot - Anti-BACE2 antibody (ab8024)
      All lanes : Anti-BACE2 antibody (ab8024) at 1 µg/ml

      Lane 1 : human heart tissue lysate with absence of blocking peptide
      Lane 2 : human heart tissue lysate with presence of blocking peptide
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACE2 antibody (ab8024)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACE2 antibody (ab8024)
      Immunohistochemistry of BACE2 in rat heart tissue with BACE2 antibody at 2 µg/ml.

    References (0)

    Publishing research using ab8024? Please let us know so that we can cite the reference in this datasheet.

    ab8024 has not yet been referenced specifically in any publications.

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