Overview

  • Product name
  • Description
    Rabbit polyclonal to BACE2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide:

    APTPGPGTPAERHADG

    , corresponding to amino acids 44-59 of Human BACE 2.

  • Positive control
    • Human heart tissue lysate.
  • General notes
    Beta-site APP Cleaving Enzyme

Properties

Applications

Our Abpromise guarantee covers the use of ab8025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Can be blocked with BACE2 peptide (ab8392).

Target

  • Function
    Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves APP, between residues 690 and 691, leading to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. It has also been shown that it can cleave APP between residues 671 and 672.
  • Tissue specificity
    Brain. Present in neurons within the hippocampus, frontal cortex and temporal cortex (at protein level). Expressed at low levels in most peripheral tissues and at higher levels in colon, kidney, pancreas, placenta, prostate, stomach and trachea. Expressed at low levels in the brain. Found in spinal cord, medulla oblongata, substantia nigra and locus coruleus. Expressed in the ductal epithelium of both normal and malignant prostate.
  • Sequence similarities
    Belongs to the peptidase A1 family.
  • Post-translational
    modifications
    Undergoes autoproteolytic cleavage.
    Glycosylated.
  • Cellular localization
    Membrane. Golgi apparatus. Endoplasmic reticulum. Endosome. Cell surface.
  • Information by UniProt
  • Database links
  • Alternative names
    • ALP 56 antibody
    • ALP56 antibody
    • Asp 1 antibody
    • ASP1 antibody
    • ASP21 antibody
    • Aspartic Like Protease 56kDa antibody
    • Aspartic-like protease 56 kDa antibody
    • Aspartyl protease 1 antibody
    • BACE 2 antibody
    • Bace2 antibody
    • BACE2_HUMAN antibody
    • Beta Secretase 2 antibody
    • Beta Site Amyloid Beta A4 Precursor Protein Cleaving Enzyme 2 antibody
    • Beta-secretase 2 antibody
    • Beta-site amyloid precursor protein cleaving enzyme 2 antibody
    • Beta-site APP cleaving enzyme 2 antibody
    • Down region aspartic protease antibody
    • Downs Syndrome Region Aspartic Protease antibody
    • DRAP antibody
    • Memapsin 1 antibody
    • Memapsin-1 antibody
    • Membrane associated aspartic protease 1 antibody
    • Membrane-associated aspartic protease 1 antibody
    • Theta-secretase antibody
    see all

Images

  • Western blot analysis of BACE2 in human (A) and mouse (B) heart tissue lysates with Ab8025 at 1 µg/ml. Western blot analysis of BACE2 in human (A) and mouse (B) heart tissue lysates with Ab8025 at 1 µg/ml.

References

This product has been referenced in:
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for contacting us and letting us know about this situation. Thank you also for your patience,as I have been in touch with the labs that produce these BACE1 and BACE2 antibodies. The specificity of the BACE1 antibody ab62034 was determined by a pathologist whovalidated the staining pattern in positive and negative control human tissues. The antibody has not been validated on knockout or wild type mouse tissues. The BACE2 antibody ab8025 wastested in Western blot for expected band size, and results were compared to other BACE2 antibodies raised against different regions of the protein. Ab8025 has also not been tested against knockout mouse samples, though it has been tested with wild type murine heart lysate. What species were your pancreas samples from? Are the staining patterns in wild type tissue expected based on literature using other antibodies?Has the BACE1 knockout been confirmed by PCR? I would recommend also trying a BACE1 wild type negative control, such as thyroid, skeletal muscle, or epidermis (BACE2 should be positive in these tissues). Since the BACE1 antibody has not been characterized in murine tissue, it is possible that it is cross-reacting, so additional tests may be necessary to determine the specificity in mice. I am sorry I don't have more data regarding the specificity of the antibodies, but I hope this information will be useful. Please let me know if you have any questions or if there is anything else that we can do for you.

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Answer

Thank you for your enquiry. The sequence does not exist as an exact match of the 16 residues, but at least four mouse proteins have enough homology with the sequence to indicate that cross-reactivity is possible, having a band of the same size. Mouse BACE2 has 9 of the same amino acids as the immunizing sequence. In this case, the matching amino acids are enough to create cross-reactivity with mouse BACE2. We hope this will help.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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