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RecombinantRabMAb

Recombinant Anti-BACE2 antibody [EPR23339-221] (ab270458)

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Immunohistochemistry (Frozen sections) - Anti-BACE2 antibody [EPR23339-221] (ab270458)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACE2 antibody [EPR23339-221] (ab270458)
  • Western blot - Anti-BACE2 antibody [EPR23339-221] (ab270458)
  • Immunoprecipitation - Anti-BACE2 antibody [EPR23339-221] (ab270458)
  • Immunocytochemistry/ Immunofluorescence - Anti-BACE2 antibody [EPR23339-221] (ab270458)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACE2 antibody [EPR23339-221] (ab270458)
  • Western blot - Anti-BACE2 antibody [EPR23339-221] (ab270458)
  • Anti-BACE2 antibody [EPR23339-221] (ab270458)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23339-221] to BACE2
  • Suitable for: IHC-Fr, WB, ICC/IF, IP, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-BACE2 antibody [EPR23339-221]
    See all BACE2 primary antibodies

  • Description

    Rabbit monoclonal [EPR23339-221] to BACE2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-Fr, WB, ICC/IF, IP, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, A549, C6, Beta-TC-6 and Neuro 2a cell lysate. IHC-P: Mouse and rat pancreas tissue. IHC-fr: Mouse pancreas. ICC/IF: Beta-TC-6 cells. IP: Neuro-2a whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab270458 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
1/100.
WB (1)
1/1000. Predicted molecular weight: 56 kDa.
ICC/IF
1/50.

100% methanol fixation is recommended.
IP
1/30.
IHC-P (1)
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
IHC-Fr
1/100.
WB
1/1000. Predicted molecular weight: 56 kDa.
ICC/IF
1/50.

100% methanol fixation is recommended.
IP
1/30.
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves APP, between residues 690 and 691, leading to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. It has also been shown that it can cleave APP between residues 671 and 672.

  • Tissue specificity

    Brain. Present in neurons within the hippocampus, frontal cortex and temporal cortex (at protein level). Expressed at low levels in most peripheral tissues and at higher levels in colon, kidney, pancreas, placenta, prostate, stomach and trachea. Expressed at low levels in the brain. Found in spinal cord, medulla oblongata, substantia nigra and locus coruleus. Expressed in the ductal epithelium of both normal and malignant prostate.

  • Sequence similarities

    Belongs to the peptidase A1 family.

  • Post-translational
    modifications

    Undergoes autoproteolytic cleavage.
    Glycosylated.

  • Cellular localization

    Membrane. Golgi apparatus. Endoplasmic reticulum. Endosome. Cell surface.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • ALP 56 antibody
    • ALP56 antibody
    • Asp 1 antibody
    • ASP1 antibody
    • ASP21 antibody
    • Aspartic Like Protease 56kDa antibody
    • Aspartic-like protease 56 kDa antibody
    • Aspartyl protease 1 antibody
    • BACE 2 antibody
    • Bace2 antibody
    • BACE2_HUMAN antibody
    • Beta Secretase 2 antibody
    • Beta Site Amyloid Beta A4 Precursor Protein Cleaving Enzyme 2 antibody
    • Beta-secretase 2 antibody
    • Beta-site amyloid precursor protein cleaving enzyme 2 antibody
    • Beta-site APP cleaving enzyme 2 antibody
    • Down region aspartic protease antibody
    • Downs Syndrome Region Aspartic Protease antibody
    • DRAP antibody
    • Memapsin 1 antibody
    • Memapsin-1 antibody
    • Membrane associated aspartic protease 1 antibody
    • Membrane-associated aspartic protease 1 antibody
    • Theta-secretase antibody
    see all

Images

  • Immunohistochemistry (Frozen sections) - Anti-BACE2 antibody [EPR23339-221] (ab270458)
    Immunohistochemistry (Frozen sections) - Anti-BACE2 antibody [EPR23339-221] (ab270458)

    Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen mouse pancreas tissue labeling BACE2 with ab270458 at 1/100 dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution. The nuclear counterstain was DAPI (Blue).

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACE2 antibody [EPR23339-221] (ab270458)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACE2 antibody [EPR23339-221] (ab270458)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labeling BACE2 with ab270458 at 1/100 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on mouse pancreatic islet (PMID: 21907142). The section was incubated with ab270458 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-BACE2 antibody [EPR23339-221] (ab270458)
    Western blot - Anti-BACE2 antibody [EPR23339-221] (ab270458)
    All lanes : Anti-BACE2 antibody [EPR23339-221] (ab270458) at 1/1000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : A549 (human lung carcinoma epithelial cell), whole cell lysate
    Lane 3 : C6 (rat glial tumor glial cell), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 56 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 136 secs.

  • Immunoprecipitation - Anti-BACE2 antibody [EPR23339-221] (ab270458)
    Immunoprecipitation - Anti-BACE2 antibody [EPR23339-221] (ab270458)

    BACE2 was immunoprecipitated from 0.35 mg Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate with ab270458 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab270458 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Neuro-2a whole cell lysate.

    Lane 2: ab270458 IP in Neuro-2a whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab270458 in Neuro-2a whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 mins.

     

  • Immunocytochemistry/ Immunofluorescence - Anti-BACE2 antibody [EPR23339-221] (ab270458)
    Immunocytochemistry/ Immunofluorescence - Anti-BACE2 antibody [EPR23339-221] (ab270458)

    Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized Beta-TC-6 cells labeling BACE2 with ab270458 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplamic and membranous staining in Beta-TC-6 cell line. 100% methanol fixation is recommended. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACE2 antibody [EPR23339-221] (ab270458)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACE2 antibody [EPR23339-221] (ab270458)

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling BACE2 with ab270458 at 1/100 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on rat pancreatic islet (PMID: 21907142). The section was incubated with ab270458 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-BACE2 antibody [EPR23339-221] (ab270458)
    Western blot - Anti-BACE2 antibody [EPR23339-221] (ab270458)
    All lanes : Anti-BACE2 antibody [EPR23339-221] (ab270458) at 1/1000 dilution

    Lane 1 : Beta-TC-6 (mouse pancreas insulinoma beta cell), whole cell lysate
    Lane 2 : Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate

    Lysates/proteins at 40 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 56 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 secs.

  • Anti-BACE2 antibody [EPR23339-221] (ab270458)
    Anti-BACE2 antibody [EPR23339-221] (ab270458)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab270458? Please let us know so that we can cite the reference in this datasheet.

ab270458 has not yet been referenced specifically in any publications.

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Western blot abreview for Anti-BACE2 antibody [EPR23339-221]

 Poor
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (cortex)
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Loading amount
20 µg
Specification
cortex
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Verified customer

Submitted Aug 23 2021

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