Overview

  • Product name

  • Description

    Rabbit polyclonal to BACH1.3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human BACH1.3 aa 350-400. The exact sequence is proprietary. NP_001177.1
    Database link: O14867

  • Positive control

    • WB: HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab245548 was affinity purified using an epitope specific to BACH1.3 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab245548 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
WB 1/2000 - 1/10000. Predicted molecular weight: 82 kDa.

Target

  • Function

    Transcriptional regulator that acts as repressor or activator. Binds, in-vitro, to NF-E2 binding sites. Play important roles in coordinating transcription activation and repression by MAFK.
  • Sequence similarities

    Belongs to the bZIP family. CNC subfamily.
    Contains 1 BTB (POZ) domain.
    Contains 1 bZIP domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • BACH 1 antibody
    • BACH 1.3 antibody
    • Bach1 antibody
    • BACH1_HUMAN antibody
    • Basic leucine zipper transcription factor 1 antibody
    • basic region leucine zipper transcriptional regulator BACH1 antibody
    • BTB and CNC homolog 1 antibody
    • BTB and CNC homology 1 antibody
    • BTB and CNC homology 1, basic leucine zipper transcription factor 1 antibody
    • HA2303 antibody
    • OTTHUMP00000096563 antibody
    • OTTHUMP00000096564 antibody
    • Transcription regulator protein BACH1 antibody
    see all

Images

  • All lanes : Anti-BACH1.3 antibody (ab245548) at 0.1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 82 kDa


    Exposure time: 3 minutes
  • BACH1.3was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245548 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab245548 at 1 µg/ml.

    Lane 1: ab245548 IP in HeLa whole cell lysate.
    Lane 2: Control IgG IP in HeLa whole cell lysate.

    Detection: Chemiluminescence with an exposure time of 10 seconds.

References

ab245548 has not yet been referenced specifically in any publications.

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