Key features and details
- Rabbit polyclonal to BACH1.3
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-BACH1.3 antibody
See all BACH1.3 primary antibodies
DescriptionRabbit polyclonal to BACH1.3
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human BACH1.3 aa 350-400. The exact sequence is proprietary. NP_001177.1
Database link: O14867
- WB: HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysate.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245548 was affinity purified using an epitope specific to BACH1.3 immobilized on solid support.
Our Abpromise guarantee covers the use of ab245548 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 82 kDa.|
FunctionTranscriptional regulator that acts as repressor or activator. Binds, in-vitro, to NF-E2 binding sites. Play important roles in coordinating transcription activation and repression by MAFK.
Sequence similaritiesBelongs to the bZIP family. CNC subfamily.
Contains 1 BTB (POZ) domain.
Contains 1 bZIP domain.
- Information by UniProt
- BACH 1 antibody
- BACH 1.3 antibody
- Bach1 antibody
All lanes : Anti-BACH1.3 antibody (ab245548) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 82 kDa
Exposure time: 3 minutes
BACH1.3was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245548 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab245548 at 1 µg/ml.
Lane 1: ab245548 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with an exposure time of 10 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab245548 has not yet been referenced specifically in any publications.