Anti-BACH1.3 antibody [OTI4E11] (ab128486)
Key features and details
- Mouse monoclonal [OTI4E11] to BACH1.3
- Suitable for: WB, IHC-P, Flow Cyt (Intra)
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-BACH1.3 antibody [OTI4E11]
See all BACH1.3 primary antibodies -
Description
Mouse monoclonal [OTI4E11] to BACH1.3 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Recombinant full length protein corresponding to Human BACH1.3 aa 1-736. produced in HEK293T cells (NP_996749).
Sequence:MSLSENSVFAYESSVHSTNVLLSLNDQRKKDVLCDVTIFVEGQRFRAHRS VLAACSSYFHSRIVGQADGELNITLPEEVTVKGFEPLIQFAYTAKLILSK ENVDEVCKCVEFLSVHNIEESCFQFLKFKFLDSTADQQECPRKKCFSSHC QKTDLKLSLLDQRDLETDEVEEFLENKNVQTPQCKLRRYQGNAKASPPLQ DSASQTYESMCLEKDAALALPSLCPKYRKFQKAFGTDRVRTGESSVKDIH ASVQPNERSENECLGGVPECRDLQVMLKCDESKLAMEPEETKKDPASQCP TEKSEVTPFPHNSSIDPHGLYSLSLLHTYDQYGDLNFAGMQNTTVLTEKP LSGTDVQEKTFGESQDLPLKSDLGTREDSSVASSDRSSVEREVAEHLAKG FWSDICSTDTPCQMQLSPAVAKDGSEQISQKRSECPWLGIRISESPEPGQ RTFTTLSSVNCPFISTLSTEGCSSNLEIGNDDYVSEPQQEPCPYACVISL GDDSETDTEGDSESCSAREQECEVKLPFNAQRIISLSRNDFQSLLKMHKL TPEQLDCIHDIRRRSKNRIAAQRCRKRKLDCIQNLESEIEKLQSEKESLL KERDHILSTLGETKQNLTGLCQKVCKEAALSQEQIQILAKYSAADCPLSF LISEKDKSTPDGELALPSIFSLSDRPPAVLPPCARGNSEPGYARGQESQQ MSTATSEQAGPAEQCRQSGGISDFCQQMTDKCTTDE
Database link: O14867 -
Positive control
- WB: HEK-293T cells transfected with BACH1.3. IHC-P: Human bladder carcinoma and tonsil tissues. Flow Cyt (Intra): BACH1 transfected HEK-293T cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 1% BSA -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant by affinity chromatography -
Clonality
Monoclonal -
Clone number
OTI4E11 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab128486 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000. Predicted molecular weight: 82 kDa.
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IHC-P |
1/150.
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Flow Cyt (Intra) |
1/100.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
1/2000. Predicted molecular weight: 82 kDa. |
IHC-P
1/150. |
Flow Cyt (Intra)
1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Transcriptional regulator that acts as repressor or activator. Binds, in-vitro, to NF-E2 binding sites. Play important roles in coordinating transcription activation and repression by MAFK. -
Sequence similarities
Belongs to the bZIP family. CNC subfamily.
Contains 1 BTB (POZ) domain.
Contains 1 bZIP domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 571 Human
- Omim: 602751 Human
- SwissProt: O14867 Human
- Unigene: 154276 Human
- Unigene: 721915 Human
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Alternative names
- BACH 1 antibody
- BACH 1.3 antibody
- Bach1 antibody
see all
Images
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All lanes : Anti-BACH1.3 antibody [OTI4E11] (ab128486) at 1/2000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK-293T cells transfected with pCMV6-ENTRY BACH1.3 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 82 kDa -
Paraffin-embedded human bladder carcinoma tissue stained for BACH1.3 using ab128486 at 1/150 dilution in immunohistochemical analysis.
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Paraffin-embedded human tonsil tissue stained for BACH1.3 using ab128486 at 1/150 dilution in immunohistochemical analysis.
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Flow cytometric analysis of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with either pCMV6-ENTRY BACH1.3 (Red) or empty vector control plasmid (Blue) stained for BACH1.3 using ab128486 at 1/100 dilution.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab128486 has been referenced in 1 publication.
- Huang X et al. Functional role of BTB and CNC Homology 1 gene in pancreatic cancer and its association with survival in patients treated with gemcitabine. Theranostics 8:3366-3379 (2018). PubMed: 29930735