Anti-BACH1.3 antibody [OTI4E11] (ab128486)

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Western blot - Anti-BACH1.3 antibody [OTI4E11] (ab128486)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACH1.3 antibody [OTI4E11] (ab128486)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACH1.3 antibody [OTI4E11] (ab128486)
  • Flow Cytometry - Anti-BACH1.3 antibody [OTI4E11] (ab128486)

Key features and details

  • Mouse monoclonal [OTI4E11] to BACH1.3
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-BACH1.3 antibody [OTI4E11]
    See all BACH1.3 primary antibodies

  • Description

    Mouse monoclonal [OTI4E11] to BACH1.3

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant full length protein corresponding to Human BACH1.3 aa 1-736. produced in HEK293T cells (NP_996749).
    Sequence:

    MSLSENSVFAYESSVHSTNVLLSLNDQRKKDVLCDVTIFVEGQRFRAHRS VLAACSSYFHSRIVGQADGELNITLPEEVTVKGFEPLIQFAYTAKLILSK ENVDEVCKCVEFLSVHNIEESCFQFLKFKFLDSTADQQECPRKKCFSSHC QKTDLKLSLLDQRDLETDEVEEFLENKNVQTPQCKLRRYQGNAKASPPLQ DSASQTYESMCLEKDAALALPSLCPKYRKFQKAFGTDRVRTGESSVKDIH ASVQPNERSENECLGGVPECRDLQVMLKCDESKLAMEPEETKKDPASQCP TEKSEVTPFPHNSSIDPHGLYSLSLLHTYDQYGDLNFAGMQNTTVLTEKP LSGTDVQEKTFGESQDLPLKSDLGTREDSSVASSDRSSVEREVAEHLAKG FWSDICSTDTPCQMQLSPAVAKDGSEQISQKRSECPWLGIRISESPEPGQ RTFTTLSSVNCPFISTLSTEGCSSNLEIGNDDYVSEPQQEPCPYACVISL GDDSETDTEGDSESCSAREQECEVKLPFNAQRIISLSRNDFQSLLKMHKL TPEQLDCIHDIRRRSKNRIAAQRCRKRKLDCIQNLESEIEKLQSEKESLL KERDHILSTLGETKQNLTGLCQKVCKEAALSQEQIQILAKYSAADCPLSF LISEKDKSTPDGELALPSIFSLSDRPPAVLPPCARGNSEPGYARGQESQQ MSTATSEQAGPAEQCRQSGGISDFCQQMTDKCTTDE


    Database link: O14867
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: HEK-293T cells transfected with BACH1.3. IHC-P: Human bladder carcinoma and tonsil tissues. Flow: BACH1 transfected HEK-293T cells.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol, 1% BSA
  • Concentration information loading...

  • Purity

    Affinity purified

  • Purification notes

    Purified from cell culture supernatant by affinity chromatography

  • Clonality

    Monoclonal

  • Clone number

    OTI4E11

  • Isotype

    IgG1

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab128486 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/2000. Predicted molecular weight: 82 kDa.
IHC-P
1/150.
Flow Cyt
1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
Notes
WB
1/2000. Predicted molecular weight: 82 kDa.
IHC-P
1/150.
Flow Cyt
1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Transcriptional regulator that acts as repressor or activator. Binds, in-vitro, to NF-E2 binding sites. Play important roles in coordinating transcription activation and repression by MAFK.

  • Sequence similarities

    Belongs to the bZIP family. CNC subfamily.
    Contains 1 BTB (POZ) domain.
    Contains 1 bZIP domain.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • BACH 1 antibody
      • BACH 1.3 antibody
      • Bach1 antibody
      • BACH1_HUMAN antibody
      • Basic leucine zipper transcription factor 1 antibody
      • basic region leucine zipper transcriptional regulator BACH1 antibody
      • BTB and CNC homolog 1 antibody
      • BTB and CNC homology 1 antibody
      • BTB and CNC homology 1, basic leucine zipper transcription factor 1 antibody
      • HA2303 antibody
      • OTTHUMP00000096563 antibody
      • OTTHUMP00000096564 antibody
      • Transcription regulator protein BACH1 antibody
      see all

    Images

    • Western blot - Anti-BACH1.3 antibody [OTI4E11] (ab128486)
      Western blot - Anti-BACH1.3 antibody [OTI4E11] (ab128486)
      All lanes : Anti-BACH1.3 antibody [OTI4E11] (ab128486) at 1/2000 dilution

      Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA
      Lane 2 : HEK-293T cells transfected with pCMV6-ENTRY BACH1.3 cDNA

      Lysates/proteins at 5 µg per lane.

      Predicted band size: 82 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACH1.3 antibody [OTI4E11] (ab128486)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACH1.3 antibody [OTI4E11] (ab128486)

      Paraffin-embedded human bladder carcinoma tissue stained for BACH1.3 using ab128486 at 1/150 dilution in immunohistochemical analysis.

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACH1.3 antibody [OTI4E11] (ab128486)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACH1.3 antibody [OTI4E11] (ab128486)

      Paraffin-embedded human tonsil tissue stained for BACH1.3 using ab128486 at 1/150 dilution in immunohistochemical analysis.

       

    • Flow Cytometry - Anti-BACH1.3 antibody [OTI4E11] (ab128486)
      Flow Cytometry - Anti-BACH1.3 antibody [OTI4E11] (ab128486)

      Flow cytometric analysis of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with either pCMV6-ENTRY BACH1.3 (Red) or empty vector control plasmid (Blue) stained for BACH1.3 using ab128486 at 1/100 dilution.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (1)

    Publishing research using ab128486? Please let us know so that we can cite the reference in this datasheet.

    ab128486 has been referenced in 1 publication.

    • Huang X  et al. Functional role of BTB and CNC Homology 1 gene in pancreatic cancer and its association with survival in patients treated with gemcitabine. Theranostics 8:3366-3379 (2018). PubMed: 29930735

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