Anti-BACH1.3 antibody [OTI4E11] (ab128486)

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Overview

  • Product name

    Anti-BACH1.3 antibody [OTI4E11]
    See all BACH1.3 primary antibodies

  • Description

    Mouse monoclonal [OTI4E11] to BACH1.3

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant full length protein corresponding to Human BACH1.3 aa 1-736. produced in HEK293T cells (NP_996749).
    Sequence:

    MSLSENSVFAYESSVHSTNVLLSLNDQRKKDVLCDVTIFVEGQRFRAHRS VLAACSSYFHSRIVGQADGELNITLPEEVTVKGFEPLIQFAYTAKLILSK ENVDEVCKCVEFLSVHNIEESCFQFLKFKFLDSTADQQECPRKKCFSSHC QKTDLKLSLLDQRDLETDEVEEFLENKNVQTPQCKLRRYQGNAKASPPLQ DSASQTYESMCLEKDAALALPSLCPKYRKFQKAFGTDRVRTGESSVKDIH ASVQPNERSENECLGGVPECRDLQVMLKCDESKLAMEPEETKKDPASQCP TEKSEVTPFPHNSSIDPHGLYSLSLLHTYDQYGDLNFAGMQNTTVLTEKP LSGTDVQEKTFGESQDLPLKSDLGTREDSSVASSDRSSVEREVAEHLAKG FWSDICSTDTPCQMQLSPAVAKDGSEQISQKRSECPWLGIRISESPEPGQ RTFTTLSSVNCPFISTLSTEGCSSNLEIGNDDYVSEPQQEPCPYACVISL GDDSETDTEGDSESCSAREQECEVKLPFNAQRIISLSRNDFQSLLKMHKL TPEQLDCIHDIRRRSKNRIAAQRCRKRKLDCIQNLESEIEKLQSEKESLL KERDHILSTLGETKQNLTGLCQKVCKEAALSQEQIQILAKYSAADCPLSF LISEKDKSTPDGELALPSIFSLSDRPPAVLPPCARGNSEPGYARGQESQQ MSTATSEQAGPAEQCRQSGGISDFCQQMTDKCTTDE


    Database link: O14867

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: HEK-293T cells transfected with BACH1.3. IHC-P: Human bladder carcinoma and tonsil tissues. Flow: BACH1 transfected HEK-293T cells.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol, 1% BSA
  • Concentration information loading...

  • Purity

    Affinity purified

  • Purification notes

    Purified from cell culture supernatant by affinity chromatography

  • Clonality

    Monoclonal

  • Clone number

    OTI4E11

  • Isotype

    IgG1

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab128486 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 82 kDa.
IHC-P 1/150.
Flow Cyt 1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Transcriptional regulator that acts as repressor or activator. Binds, in-vitro, to NF-E2 binding sites. Play important roles in coordinating transcription activation and repression by MAFK.

  • Sequence similarities

    Belongs to the bZIP family. CNC subfamily.
    Contains 1 BTB (POZ) domain.
    Contains 1 bZIP domain.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • BACH 1 antibody
      • BACH 1.3 antibody
      • Bach1 antibody
      • BACH1_HUMAN antibody
      • Basic leucine zipper transcription factor 1 antibody
      • basic region leucine zipper transcriptional regulator BACH1 antibody
      • BTB and CNC homolog 1 antibody
      • BTB and CNC homology 1 antibody
      • BTB and CNC homology 1, basic leucine zipper transcription factor 1 antibody
      • HA2303 antibody
      • OTTHUMP00000096563 antibody
      • OTTHUMP00000096564 antibody
      • Transcription regulator protein BACH1 antibody
      see all

    Images

    • Western blot - Anti-BACH1.3 antibody [OTI4E11] (ab128486)
      Western blot - Anti-BACH1.3 antibody [OTI4E11] (ab128486)
      All lanes : Anti-BACH1.3 antibody [OTI4E11] (ab128486) at 1/2000 dilution

      Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA
      Lane 2 : HEK-293T cells transfected with pCMV6-ENTRY BACH1.3 cDNA

      Lysates/proteins at 5 µg per lane.

      Predicted band size: 82 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACH1.3 antibody [OTI4E11] (ab128486)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACH1.3 antibody [OTI4E11] (ab128486)

      Paraffin-embedded human bladder carcinoma tissue stained for BACH1.3 using ab128486 at 1/150 dilution in immunohistochemical analysis.

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACH1.3 antibody [OTI4E11] (ab128486)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACH1.3 antibody [OTI4E11] (ab128486)

      Paraffin-embedded human tonsil tissue stained for BACH1.3 using ab128486 at 1/150 dilution in immunohistochemical analysis.

       

    • Flow Cytometry - Anti-BACH1.3 antibody [OTI4E11] (ab128486)
      Flow Cytometry - Anti-BACH1.3 antibody [OTI4E11] (ab128486)

      Flow cytometric analysis of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with either pCMV6-ENTRY BACH1.3 (Red) or empty vector control plasmid (Blue) stained for BACH1.3 using ab128486 at 1/100 dilution.

    References

    This product has been referenced in:

    • Huang X  et al. Functional role of BTB and CNC Homology 1 gene in pancreatic cancer and its association with survival in patients treated with gemcitabine. Theranostics 8:3366-3379 (2018). Read more (PubMed: 29930735) »
    See 1 Publication for this product

    Publishing research using ab128486? Please let us know so that we can cite the reference in this datasheet.

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