Anti-BACH1/BRIP1 antibody (ab49657)
Key features and details
- Rabbit polyclonal to BACH1/BRIP1
- Suitable for: WB, ICC
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-BACH1/BRIP1 antibody
See all BACH1/BRIP1 primary antibodies -
Description
Rabbit polyclonal to BACH1/BRIP1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICCmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide:
NFKPSPSKNKGMFPGFK
conjugated to KLH by a N terminal Cysteine residue linker, corresponding to amino acids 1233-1249 of Human BACH1/BRIP1 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab49657 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 141 kDa.
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ICC |
Use a concentration of 10 - 20 µg/ml.
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Notes |
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WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 141 kDa. |
ICC
Use a concentration of 10 - 20 µg/ml. |
Target
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Function
DNA-dependent ATPase and 5' to 3' DNA helicase required for the maintenance of chromosomal stability. Acts late in the Fanconi anemia pathway, after FANCD2 ubiquitination. Involved in the repair of DNA double-strand breaks by homologous recombination in a manner that depends on its association with BRCA1. -
Tissue specificity
Ubiquitously expressed, with highest levels in testis. -
Involvement in disease
Defects in BRIP1 are a cause of susceptibility to breast cancer (BC) [MIM:114480]. A common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and genetically heterogeneous. Important genetic factors have been indicated by familial occurrence and bilateral involvement. Mutations at more than one locus can be involved in different families or even in the same case.
Defects in BRIP1 are the cause of Fanconi anemia complementation group J (FANCJ) [MIM:609054]. It is a disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair. -
Sequence similarities
Belongs to the DEAD box helicase family. DEAH subfamily.
Contains 1 helicase ATP-binding domain. -
Domain
4Fe-4S iron-sulfur-binding is required for helicase activity (PubMed:20639400). -
Post-translational
modificationsPhosphorylated. Phosphorylation is necessary for interaction with BRCA1, and is cell-cycle regulated. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 83990 Human
- Omim: 605882 Human
- SwissProt: Q9BX63 Human
- Unigene: 128903 Human
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Alternative names
- ATP dependent RNA helicase BRIP1 antibody
- ATP-dependent RNA helicase BRIP1 antibody
- BACH 1 antibody
see all
Images
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Immunocytochemistry analysis of MCF-7 cells labeling BACH1/BRIP1 with ab49657 at 20 μg/mL followed by Goat Anti-Rabbit IgG (whole molecule)-FITC secondary antibodyat 1/100. Cells were fixed and permeabilized with 4% paraformaldehyde. Panel C: cells were counterstained with DAPI (blue) to stain nuclei (Panel A: without DAPI, Panel B: only DAPI).
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Anti-BACH1/BRIP1 antibody (ab49657) at 1/2000 dilution + MCF-7 cell lysate
Secondary
HRP conjugated Anti-Rabbit IgG antibody
Developed using the ECL technique.
Predicted band size: 141 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab49657 has been referenced in 9 publications.
- Chen Y et al. Bushen Culuan Decoction Ameliorates Premature Ovarian Insufficiency by Acting on the Nrf2/ARE Signaling Pathway to Alleviate Oxidative Stress. Front Pharmacol 13:857932 (2022). PubMed: 35462905
- Jian L et al. Haem relieves hyperoxia-mediated inhibition of HMEC-1 cell proliferation, migration and angiogenesis by inhibiting BACH1 expression. BMC Ophthalmol 21:104 (2021). PubMed: 33632168
- Li D et al. Expression of nuclear factor erythroid-2-related factor 2, broad complex-tramtrack-bric a brac and Cap'n'collar homology 1 and ?-glutamic acid cysteine synthase in peripheral blood of patients with chronic obstructive pulmonary disease and its clinical significance. Exp Ther Med 21:516 (2021). PubMed: 33815589
- K JCB et al. Loss of Mitochondrial Localization of Human FANCG Causes Defective FANCJ Helicase. Mol Cell Biol 40:N/A (2020). PubMed: 32989015
- Hu Z et al. BGL3 lncRNA mediates retention of the BRCA1/BARD1 complex at DNA damage sites. EMBO J 39:e104133 (2020). PubMed: 32347575
- Li YY et al. Protective effects of HO-1 pathway on lung injury subsequent to limb ischemia reperfusion. Kaohsiung J Med Sci 35:417-424 (2019). PubMed: 30977589
- Li X et al. Inhibition of AZIN2-sv induces neovascularization and improves prognosis after myocardial infarction by blocking ubiquitin-dependent talin1 degradation and activating the Akt pathway. EBioMedicine N/A:N/A (2018). PubMed: 30545799
- Liu Y & Zheng Y Bach1 siRNA attenuates bleomycin-induced pulmonary fibrosis by modulating oxidative stress in mice. Int J Mol Med 39:91-100 (2017). WB . PubMed: 27959382
- Nath S et al. FANCJ helicase controls the balance between short- and long-tract gene conversions between sister chromatids. Nucleic Acids Res 45:8886-8900 (2017). PubMed: 28911102