Overview

  • Product name

    Anti-BACH1/BRIP1 antibody [EPR7914(2)]
    See all BACH1/BRIP1 primary antibodies
  • Description

    Rabbit monoclonal [EPR7914(2)] to BACH1/BRIP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human BACH1/BRIP1 aa 400-500. The exact sequence is proprietary.
    Database link: Q9BX63

  • Positive control

    • HEK293T, HeLa and Jurkat whole cell lysate (ab7899)
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab180853 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 141 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function

      DNA-dependent ATPase and 5' to 3' DNA helicase required for the maintenance of chromosomal stability. Acts late in the Fanconi anemia pathway, after FANCD2 ubiquitination. Involved in the repair of DNA double-strand breaks by homologous recombination in a manner that depends on its association with BRCA1.
    • Tissue specificity

      Ubiquitously expressed, with highest levels in testis.
    • Involvement in disease

      Defects in BRIP1 are a cause of susceptibility to breast cancer (BC) [MIM:114480]. A common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and genetically heterogeneous. Important genetic factors have been indicated by familial occurrence and bilateral involvement. Mutations at more than one locus can be involved in different families or even in the same case.
      Defects in BRIP1 are the cause of Fanconi anemia complementation group J (FANCJ) [MIM:609054]. It is a disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair.
    • Sequence similarities

      Belongs to the DEAD box helicase family. DEAH subfamily.
      Contains 1 helicase ATP-binding domain.
    • Domain

      4Fe-4S iron-sulfur-binding is required for helicase activity (PubMed:20639400).
    • Post-translational
      modifications

      Phosphorylated. Phosphorylation is necessary for interaction with BRCA1, and is cell-cycle regulated.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • ATP dependent RNA helicase BRIP1 antibody
      • ATP-dependent RNA helicase BRIP1 antibody
      • BACH 1 antibody
      • BRAC 1 Associated C Terminal Helicase 1 antibody
      • BRCA 1 Interacting Protein 1 antibody
      • BRCA1 binding helicase like protein BACH1 antibody
      • BRCA1 interacting protein C terminal helicase 1 antibody
      • BRCA1-associated C-terminal helicase 1 antibody
      • BRCA1-interacting protein 1 antibody
      • BRCA1-interacting protein C-terminal helicase 1 antibody
      • BRCA1/BRCA2 associated helicase 1 antibody
      • BRIP 1 antibody
      • BRIP1 antibody
      • FANCJ antibody
      • FANCJ_HUMAN antibody
      • Fanconi anemia group J protein antibody
      • FLJ90232 antibody
      • MGC126521 antibody
      • MGC126523 antibody
      • OF antibody
      • Protein FACJ antibody
      see all

    Images

    • All lanes : Anti-BACH1/BRIP1 antibody [EPR7914(2)] (ab180853) at 1/1000 dilution

      Lane 1 : HEK293T cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 141 kDa
      Observed band size: 105 kDa
      why is the actual band size different from the predicted?

    References

    This product has been referenced in:

    • Maio N  et al. Dimeric ferrochelatase bridges ABCB7 and ABCB10 homodimers in an architecturally defined molecular complex required for heme biosynthesis. Haematologica N/A:N/A (2019). Read more (PubMed: 30765471) »
    See 1 Publication for this product

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