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I am interested in how this antibody was generated. What dosage of immunogen did the rabbit receive and how frequently. Any more information on the immunogen would be helpful too. Thank you in advance!
Asked on Sep 24 2012
Thank you for contacting Abcam regarding ab13808.
Regarding the production of this antibody, the immunogen is a synthetic peptide comprised of amino acids 240-254 of the Anthrax PA protein. No further details are available at this time.
Antibody production utilizes a 10 week protocol summarized below. Further details are considered proprietary and not available.
1st Week: Pre-immunization bleed (5 mL sera/rabbit) and 1st immunization
2nd Week: 2nd immunization
3rd Week: 3rd immunization
4th Week: 4th immunization
5th Week: 5th immunization
6th Week: 1st production bleed (10˜15 mL sera/per rabbit)
7th Week: 6th immunization
8th Week: 2nd production bleed (10˜15 mL sera/per rabbit)
9th Week: 7th immunization
10th Week: 3rd production bleed (10˜15 mL sera/per rabbit)
I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions.
Answered on Sep 24 2012