Overview

  • Product name
    Anti-Bacillus anthracis protective antigen antibody [C3]
    See all Bacillus anthracis protective antigen primary antibodies
  • Description
    Mouse monoclonal [C3] to Bacillus anthracis protective antigen
  • Host species
    Mouse
  • Specificity
    Protective antigen of Bacillus anthracis Does not cross-react with with lethal factor antigen of B. antracis, Y.Pestis, F. Tularensis, Toxoplasma gondii
  • Tested applications
    Suitable for: Sandwich ELISA, ELISAmore details
  • Immunogen

    Highly purified protective antigen of Bacillus anthracis.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer
    Preservative: 0.1% Sodium azide
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    Purified by chromatography on protein A Sepharose. Purity is tested by electrophoresis.
  • Clonality
    Monoclonal
  • Clone number
    C3
  • Myeloma
    Sp2/0
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8240 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution. Can be paired for ELISA with Mouse monoclonal [BAP0102] to Bacillus anthracis protective antigen (HRP) (ab1990). Can be used as capture antibody

Target

  • Relevance
    Anthrax toxins are composed of three distinct proteins, a protective antigen (PA), a lethal factor (LF) and an edema factor (EF). None of these proteins are toxic by themselves and several studies indicate that the anthrax toxin has the familiar A-B enzymatic binding structure, with PA acting as the binding domain and EF and/or LF acting as the active fragments.
  • Cellular localization
    Secreted
  • Alternative names
    • PA antibody
    • PA83 antibody
    • pag antibody
    • PagA antibody
    • Protective antigen antibody
    see all

References

This product has been referenced in:
See 1 Publication for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Answer

Thank you for your inquiry. Unfortunately, we do not have any anthrax PA antigen available in our catalog. I would like to recommend checking the Biocompare website which has an excellent antibody search facility that includes many suppliers. The link is: www.biocompare.com I hope this information is nevertheless helpful to you. Please do not hesitate to contact me if you have any further questions in this regard.

Read More

Answer

Thank you for contacting us. Our antibody ab21267 is against Anthrax EF, it is not an anti-Anthrax PA. We have a pair of antibodies anti-Anthrax PA for sELISA : - ab8240 that can be used as capture antibody - ab1990 that can be used as detection antibody (conjugated HRP) I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Answer

Thank you for your enquiry. We unfortunately do not have information on the exact domains that these antibodies bind to. For clones BAP0101-BAP0106 all recognize both full size (83kDa) and cleaved (63kDa)protective antigen. The antibodies can be divided in 3 groups based on the epitopes they recognize, all groups have different pattern of protein band staining in Western blotting. Group 1: BAP0101, BAP0102, BAP0106 Group 2: BAP0105 Group 3: BAP0103, BAP0104 If I can obtain any additional information, I will let you know. Have a great weekend!

Read More

Answer

Thank you for your enquiry. Ab8240 has only to our knowledge been tested for application in ELISA and Western blotting, and has not yet been tested for application in immunomicroscopy or any other application. I am thus unable to recommend a specific dilution, and could only suggest a range of 1:20 to 1:200 with optimization based upon results. Ab6785 is an appropriate secondary antibody to use along with ab8240, however. If you decide to go ahead and purchase this product, please let us know how you get on and in return we will award you 50 points with the Abcam Loyalty Scheme which can be redeemed on a number of rewards. Our prices include not just the antibody, but excellent technical support and customer service. If you have any additional questions, please contact us again.

Read More

Answer

Thank you for your enquiry. Ab8215 binds to the toxin binding protein (protective antigen). For ab8240 and ab8241, unfortunately the epitopes have not been determined. Ab8240 and ab8241 are forming a pair in sandwich ELISA (ab8240 as the capture antibody and ab8241 as detection antibody). The clones are not competing with each other.

Read More

Answer

Unfortunately all information that we have available is that the clones recognize different epitopes of the protective antigen. We are not offering the antigen.

Read More

Answer

Our best reccomended pair for EIA of BA protective antigen is pair ab8242 (coating) - ab8243. Protocol for assay as well as example of calibration curve can be found on the online datasheets for these two products. We are only able to offer these products as stated on the datasheet.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up