Overview

  • Product name
    Anti-Bacillus cereus antibody
  • Description
    Rabbit polyclonal to Bacillus cereus
  • Host species
    Rabbit
  • Specificity
    Reacts with spores and vegetative cells of Bacillus cereus and Bacillus subtilis. Antiserum is unabsorbed and may cross-react with other Bacillus species.
  • Tested applications
    Suitable for: ICC/IFmore details
  • Species reactivity
    Bacillus cereus Bacillus subtilis
  • Immunogen

    Purified spores of Bacillus cereus (ATCC 11778) and Bacillus subtilis (ATCC 9372).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.1% Sodium azide
    Constituent: 0.0268% PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab20556 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.

Target

  • Relevance
    Bacillus cereus is a Gram-positive, facultatively aerobic sporeformer whose cells are large rods and whose spores do not swell the sporangium. These and other characteristics, including biochemical features, are used to differentiate and confirm the presence B. cereus, although these characteristics are shared with B. cereus var. mycoides, B. thuringiensis and B. anthracis. Differentiation of these organisms depends upon determination of motility (most B. cereus are motile), presence of toxin crystals (B. thuringiensis), hemolytic activity (B. cereus and others are beta hemolytic whereas B. anthracis is usually nonhemolytic), and rhizoid growth which is characteristic of B. cereus var. mycoides.
  • Cellular localization
    Bacterial spore
  • Alternative names
    • B. cereus antibody
    • B.cereus antibody

References

ab20556 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-9 of 9 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Bacteria Cell (Endospore)
Permeabilization
No
Specification
Endospore
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Fixative
Glutaraldehyde

Mr. Alan Cookson

Verified customer

Submitted Jul 14 2015

Answer

Thank you for your enquiry.

This is to confirm that the status of this Abreview has been changed from edited to submitted. It will be processed shortly by a member of our Scientific Team.

If you need any further assistance in the future, please do not hesitate to contact me.

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Answer

Thank you for your response.

This is to confirm that the two other Abreviews have been published and the Discount codes are active. As soon as the third Abreview is submitted, its Discount code will be activated as well.

Good luck with the experiments and I am looking forward to receiving the Abreview.

If you need any further assistance in the future, please do not hesitate to contact me.

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Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (porcine plasma-based in vitro grown biofilm)
Specification
porcine plasma-based in vitro grown biofilm
Fixative
Paraformaldehyde
Antigen retrieval step
Other
Permeabilization
No
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Nov 08 2012

Answer

Thank you for your enquiry.

I have changed the status from edited to submitted of these two Abreviews (ab19980 and ab20556). They have come through to our internal system without any problem so they will be processed very shortly by one of the members of our Scientific Team.

However, it seems that the other Abreview ab68538 does not have the “Rating” information and the system recognizes it as a potential problem. I have resent this to you and ask you please look through all the information and resubmit it.

Thank you for your co-operation and I look forward to receiving your Abreviews.

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Answer

DISCOUNT CODES:

ab19980 - 100%ABR-E76ZR
ab20556 - 100%ABR-E76ZL
ab68538 - 100%ABR-E7657




Expiration date: 12/11/2012

I am very pleased to hear you would like to accept our offer and testab19980, ab20556 and ab68538in IHC-P.

This code will give you: 1 per discount code free PRIMARY ANTIBODY OFF OF ORDER before the expiration date. To redeem this offer, please submit an Abreview for IHC-P and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Question

I would like to test Anti-Enterococcus antibody (ab19980) in IHC-P application on biofilm and minipig tissue samples. But in the same experiment with same samples, I plan to detect Bacillus bacteria with Anti-Bacillus cereus antibody (ab20556) and Pseudomonas bacteria with Anti-Pseudomonas antibody (ab68538) also which are both not tested in IHC-P application yet. (And the fourth antibody to be ordered is Anti-Staphylococcus aureus antibody [704] (ab 37644) which is already IHC-P aprooved.)

It is much more simple and less time consuming to test all three mentionned antibodies in the same run in order to develop and implement the optimal staining protocol for our laboratory, but what if I order the antibodies separatelly what is profitable for me if discount will be asigned with each order even over paying the shipping 3 times?

I’ll be greatfull, If I could get a discount for all Abs what you probably understand.

It depends on what is more valuable for your company, whether money or application information.

Thanks for an answer in advance.



Best regards

Jan






















Jan Kučera, MSc.
Histologist
Laboratory for wound healing









Mobile: +420605969 129
Phone: +420465519 571
E-mail:jan.kucera@contipro.com









Contipro Pharma a.s.
561 02 Dolní Dobrouč, Czech Republic
http://www.contipro.com


































From: technical@abcam.com [mailto:technical@abcam.com]
Sent: Wednesday, July 04, 2012 3:05 PM
To: Kučera Jan
Subject: Reply from Abcam to your enquiry regarding ab69232, ab107092 [CCE3921219]



















https://www.abcam.com/index.html?utm_campaign=CRM&utm_source=Abcam.CRM&utm_medium=Email&utm_term=Header









Dear Jan

Thank you for your interest.

Normally, we can offer one discount code for testing at a time for one product only not for multiple products. Please do let me know which product you wish to test first and confirm the product code number and the application/species.

If you need any further assistance in the future, please do not hesitate to contact me.






Best regards,
Tanya

Tanya Bagrij, PhD
Scientific Support Supervisor
Abcam plc
www.abcam.com


Your original inquiry to Abcam:



Hallo Tanya,

Thank you for your response and information you’ve sent me.

I would like to continue in our previous correspondence regarding testing the antibodies in yet untested application.

After reading the terms and conditions at the web site you have mailed me, I would like to ask you some questions to understand the terms correctly.



Terms and conditions:

1. Limited number of discounts available, Abcam reserves the right to select candidates for this offer

2. Code must be redeemed within four months after issue date

3. Code is valid after submission of the Abreview to Abcam. All required fields of the Abreview must be completed

4. The discount will be valid for one order

5. This offer cannot be used in conjunction with other promotions. Credit notes for previous purchases can be redeemed against purchases in this promotion

6. Limit one discount code per primary antibody and protein purchased

7. Offer applies to primary antibodies and proteins

8. The discount code must be redeemed for the same product type as originally purchased e.g. primary antibody for primary antibody, protein for protein



Regarding article 1: I would like to order 3 yet untested primary antibodies (ab68538, ab19980 and ab20556) and one allready tested (ab37644). Will I get 3 discount codes?

Regarding article 4: Meaning one future order of any primary antibody in the same number as the number of discount code/s acquired?



If the assigning of discount codes depends on procedures resp. protocols I would like to test, please, do not hesitate to contact me and I will e-mail them by return.



Best regard







Abcam Customer Services and Scientific Support Team
https://www.abcam.com/index.html?pageconfig=technical&utm_campaign=CRM&utm_source=Abcam.CRM&utm_medium=Email&utm_term=Body

[CCE3921219]













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Answer

Thank you for your response and your interest.

It is very unusual to offer a discount code for more than 1 product particularly when the application is not one of the standard immunoassays (Western blot, ELISA, Flow cytometry, Immunohistochemistry etc).

The technique you wish to use/test our products is not the classical IHC-P (formalin-fixed paraffin embedded immunohistochemistry) procedure. At this stage it is not know how frequently other customers will use these products in such an assay and how easy to modify the technique.

Could you please confirm how the tissue sections will be processed and fixed and whether you have any publication on this specific technique? We need to consider carefully the applicability of this assay.

I look forward to hearing from you soon.

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Answer

Dear Ms. Kennett, Thank you for your enquiry. This is a polyclonal antibody and therefore it is recognizing multiple epitopes on multiple proteins. The exact proteins recognized have not been identified. When tested for immunofluorescence, reactivity with surface proteins is visible. Unfortunately, due to the small size of the cells and spores, it is difficult to see if intracellular staining may also occur. I hope that this information helps. Please don't hesitate to contact us for advice in the future.

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Answer

Thank you for your enquiry. In answer to your question rab20556 is reactive with both spores and vegetative cells of Bacillus cereus and Bacillus subtilis. This testing was done in IFA. I hope this information helps.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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