Anti-Bacillus thuringiensis CRY1Ab Toxin antibody (ab51586)

Submit a review Q&A (2)References (4)

Key features and details

  • Rabbit polyclonal to Bacillus thuringiensis CRY1Ab Toxin
  • Suitable for: IP, WB, ELISA
  • Isotype: IgG

Overview

  • Product name

    Anti-Bacillus thuringiensis CRY1Ab Toxin antibody

  • Description

    Rabbit polyclonal to Bacillus thuringiensis CRY1Ab Toxin

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, WB, ELISAmore details

  • Species reactivity

    Reacts with: Other species

  • Immunogen

    Full length native protein (purified) corresponding to Bacillus thuringiensis CRY1Ab Toxin.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.30
    Constituent: PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab51586 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 131 kDa.
ELISA 1/500 - 1/800.

Target

  • Relevance

    CRY1A belongs to the delta endotoxin family and is one of the most studied insecticidal proteins produced by Bacillus thuringiensis. The crystal protein is produced during sporulation and is accumulated both as an inclusion and as part of the spore coat. The toxic segment of the protein is located in the N-terminus. Promotes colloidosmotic lysis by binding to the midgut epithelial cells of many lepidopteran larvae.

  • Alternative names

    • 130 kDa crystal protein antibody
    • bt2 antibody
    • cry 1 2 antibody
    • cry1A(b) antibody
    • cryIA(b) antibody
    • cryIC1 antibody
    • Crystaline entomocidal protoxin antibody
    • Insecticidal delta endotoxin CryIA(b) antibody
    • Pesticidal crystal protein cry1Ab antibody
    see all

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (4)

Publishing research using ab51586? Please let us know so that we can cite the reference in this datasheet.

ab51586 has been referenced in 4 publications.

  • Ding L  et al. Laboratory evaluation of transgenic Populus davidiana×Populus bolleana expressing Cry1Ac + SCK, Cry1Ah3, and Cry9Aa3 genes against gypsy moth and fall webworm. PLoS One 12:e0178754 (2017). PubMed: 28582405
  • Rocha LO  et al. Multi-Method Approach for Characterizing the Interaction between Fusarium verticillioides and Bacillus thuringiensis Subsp. Kurstaki. PLoS One 9:e92189 (2014). IF . PubMed: 24739804
  • Gu K  et al. Production of marker-free transgenic Jatropha curcas expressing hybrid Bacillus thuringiensis d-endotoxin Cry1Ab/1Ac for resistance to larvae of tortrix moth (Archips micaceanus). Biotechnol Biofuels 7:68 (2014). WB ; Rice . PubMed: 24808924
  • Yang H  et al. Weak transcription of the cry1Ac gene in nonsporulating Bacillus thuringiensis cells. Appl Environ Microbiol 78:6466-74 (2012). WB . PubMed: 22773626

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Question

Good morning,

I would like to know if you can produce two monoclonal antibodies antidobies, but that these recognize different epitopes of the cry1Ab toxin.



Thanks.

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Answer

Thank you for contacting us and your interest in our products.

We currently have two antibodies available in our catalogue directed against the cry1Ab toxin. One is a rabbit polyclonal antibody (ab51586) raised against purified Bacillus thuringiensis CRY1Ab protein, the other a mouse monoclonal (ab113679) raised against the Bacillus thuringiensis Cry1Ab Toxin /Cry1Ac fusion protein.

As the rabbit polyclonal antibody ab51586 is raised against the whole length protein we would not be able to say which part of the proteins is recognised. However, the mouse monoclonal (ab113679) has been raised against a peptide taken from within the residues 400 to 440 of Bacillus thuringiensis CRY1Ab Toxin.

Unfortunately, we are not able to provide custom antibody synthesis at present, but our sister company Epitomics may be able to help. The specialize in making rabbit monoclonal antibodies. They can be reached on the following email: mailto:service@epitomics.com

I hope this information has been of help. If I can be of any further assistance, please do not hesitate to ask.

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Question

The cat no is Ab51586.

Application is Elisa .

Regards

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Answer

Thank you for your reply.

I am sorry to confirm that at this time we haven’t tested ab51586Bacillus thuringiensis CRY1Ab Toxin antibodyfor detection of fusion cotton gene.

I'm sorry I couldn't give you positive news on this occasion. If you have any further questions,please do not hesitate to contact us.

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