Anti-Bacillus thuringiensis CRY1Ab Toxin antibody (ab51586)
Key features and details
- Rabbit polyclonal to Bacillus thuringiensis CRY1Ab Toxin
- Suitable for: IP, WB, ELISA
- Isotype: IgG
Overview
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Product name
Anti-Bacillus thuringiensis CRY1Ab Toxin antibody -
Description
Rabbit polyclonal to Bacillus thuringiensis CRY1Ab Toxin -
Host species
Rabbit -
Tested applications
Suitable for: IP, WB, ELISAmore details -
Species reactivity
Reacts with: Other species -
Immunogen
Full length native protein (purified) corresponding to Bacillus thuringiensis CRY1Ab Toxin.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab51586 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
Use at an assay dependent concentration.
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| WB |
Use at an assay dependent concentration. Predicted molecular weight: 131 kDa.
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| ELISA |
1/500 - 1/800.
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| Notes |
|---|
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IP
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 131 kDa. |
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ELISA
1/500 - 1/800. |
Target
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Relevance
CRY1A belongs to the delta endotoxin family and is one of the most studied insecticidal proteins produced by Bacillus thuringiensis. The crystal protein is produced during sporulation and is accumulated both as an inclusion and as part of the spore coat. The toxic segment of the protein is located in the N-terminus. Promotes colloidosmotic lysis by binding to the midgut epithelial cells of many lepidopteran larvae. -
Alternative names
- 130 kDa crystal protein antibody
- bt2 antibody
- cry 1 2 antibody
see all
Protocols
Datasheets and documents
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Datasheet download
References (4)
ab51586 has been referenced in 4 publications.
- Ding L et al. Laboratory evaluation of transgenic Populus davidiana×Populus bolleana expressing Cry1Ac + SCK, Cry1Ah3, and Cry9Aa3 genes against gypsy moth and fall webworm. PLoS One 12:e0178754 (2017). PubMed: 28582405
- Rocha LO et al. Multi-Method Approach for Characterizing the Interaction between Fusarium verticillioides and Bacillus thuringiensis Subsp. Kurstaki. PLoS One 9:e92189 (2014). IF . PubMed: 24739804
- Gu K et al. Production of marker-free transgenic Jatropha curcas expressing hybrid Bacillus thuringiensis d-endotoxin Cry1Ab/1Ac for resistance to larvae of tortrix moth (Archips micaceanus). Biotechnol Biofuels 7:68 (2014). WB ; Rice . PubMed: 24808924
- Yang H et al. Weak transcription of the cry1Ac gene in nonsporulating Bacillus thuringiensis cells. Appl Environ Microbiol 78:6466-74 (2012). WB . PubMed: 22773626
