Overview

  • Product name

    Anti-bacterial Alkaline Phosphatase antibody (Biotin)
  • Description

    Rabbit polyclonal to bacterial Alkaline Phosphatase (Biotin)
  • Host species

    Rabbit
  • Conjugation

    Biotin
  • Tested applications

    Suitable for: IHC-Fr, Immunomicroscopy, WB, Dot blot, ELISAmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Full length protein (E. coli).

  • General notes

    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per rabbit IgG molecule.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.60
    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
  • Concentration information loading...
  • Purity

    IgG fraction
  • Purification notes

    This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab7321 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
Immunomicroscopy
WB
Dot blot
ELISA
  • Application notes
    Immunoblotting (western or dot blot)
    ELISA (1:4,000 to 1:20,000)
    Immuno microscopy
    Immunohistochemistry
    as well as other peroxidase-antibody based enzymatic assays.
  • Target

    • Relevance

      Bacterial alkaline phosphatase (also known as PhoA) is derived from E. coli. It appears to require export to the periplasm to show enzymatic activity. Fusion of the secreted alkaline phosphatase to an integral cytoplasmic membrane protein of E. coli shows different activities depending on where with the membrane protein the alkaline phosphatase is fused. Consequently, analysis of alkaline phosphatase fusions to membrane proteins of unknown structure are generally useful in determining their membrane topologies.
    • Cellular localization

      Periplasm
    • Alternative names

      • Alkaline phosphatase antibody
      • APase antibody
      • BAP antibody
      • PhoA antibody

    References

    ab7321 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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