For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    bacterial-viability-assay-kit-ab189818.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Kits/ Lysates/ Other Kits Apoptosis Kits Apoptosis Assay Kits Cell viability
Share by email

Bacterial viability assay kit (ab189818)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Submit a question References (2)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

E. coli JM109 staining.

    Key features and details

    • Platform: Microplate reader, Fluorescence microscope
    • Assay time: 2 hr 30 min

    You may also be interested in

    Biochemical
    Product image
    L-AP4, group III mGlu agonist (ab120002)
    ELISA
    Product image
    BrdU Cell Proliferation ELISA Kit (colorimetric) (ab126556)
    Primary
    Product image
    Anti-PML Protein antibody [EPR16792] (ab179466)

    View more associated products

    Overview

    • Product name

      Bacterial viability assay kit
      See all Cell Viability kits
    • Assay time

      2h 30m
    • Product overview

      Bacterial viability assay kit - (ab189818) utilizes two highly-specific, ultrasensitive fluorescent reagents to quickly and easily assess the percentage of live and dead cells within a bacterial culture. The total cell stain is permeant to all cells and thus all bacteria within the culture will be stained allowing the total number to be calculated. The dead cell stain is inpermeant to living cells and as such will only be able to enter and stain dead cells; this allows the number of dead cells to be calculated. The ratio of dead to live cells can then be quickly and easily calculated. 


      Although only validated with E. coli, results should be obtainable with a wide range of both gram positive and gram negative bacterial species including Agrobacterium tumefaciens, Pseudomonas sp and Bacillus sp.


      Excitation wavelength (nm): 490/536


      Emission wavelength (nm): 525/617


      Bacterial viability assay protocol summary:
       - harvest cells by spinning at 10,000 g for 10 min and resuspend in buffer
      - incubate for 1 hr
      - spin at 10,000 g for 10 min, resuspend and repeat
      - add total cell stain and dead cell stain
      - either incubate for 1 hr and analyze with microplate reader or incubate for 15 min and analyze with fluorescence microscope

    • Notes

      The ability to measure the viability of a bacterial culture has importance in many aspects of microbiology. Whether dealing with uncharacterized species or pathogenic strains, the ability to measure the effects of different media or the effect of drug treatment on bacterial survival is highly advantageous in advancing knowledge of these organisms.

      Kit size: 100 tests including dead cell controls using 1 µL Total Cell Stain and 1 µL Dead Cell stain for 1 mL sample.

    • Platform

      Microplate reader, Fluorescence microscope

    Properties

    • Storage instructions

      Please refer to protocols.
    • Components 100 tests
      Dead Cell Stain 1 x 0.2ml
      Total Cell Stain 1 x 0.2ml
      Wash Buffer 2 x 30ml
    • Research areas

      • Kits/ Lysates/ Other
      • Kits
      • Apoptosis Kits
      • Apoptosis Assay Kits
      • Cell viability
      • Kits/ Lysates/ Other
      • Kits
      • Cell Damage Kits
      • Cell viability, plasma membrane damage
    • Relevance

      Cell viability is a determination of living or dead cells, based on a total cell population. Cell viability assess healthy cells in a sample, with no distinction between dividing or quiescent cells. An increase in cell viability indicates cell growth, while a decrease in viability can generally be interpreted as the result of either toxic effects of compounds/agents or suboptimal culture conditions.
    • Alternative names

      • cell tracking

    Associated products

    • Related Products

      • Acid Fast Bacteria (AFB) Stain Kit (Microorganism Stain) (ab150660)
      • Live and Dead Cell Staining Kit (ab65470)

    Images

    • E. coli JM109 staining.
      E. coli JM109 staining.

      E. coli JM109 were stained as described using the protocols, and fluorescence was measured using a plate reader and a microscope. Number of stained cells were quantified

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (2)

    Publishing research using ab189818? Please let us know so that we can cite the reference in this datasheet.

    ab189818 has been referenced in 2 publications.

    • Vieira D  et al. Engineering surgical stitches to prevent bacterial infection. Sci Rep 12:834 (2022). PubMed: 35039588
    • Grogan C  et al. An evaluation of fusion partner proteins for paratransgenesis in Asaia bogorensis. PLoS One 17:e0273568 (2022). PubMed: 36048823

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab189818.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2023 Abcam plc. All rights reserved.