Key features and details
- Rabbit polyclonal to Bacteriophage PhiC31 Integrase
- Suitable for: WB, ELISA
- Isotype: IgG
Product nameAnti-Bacteriophage PhiC31 Integrase antibody
DescriptionRabbit polyclonal to Bacteriophage PhiC31 Integrase
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Other species
Synthetic peptide corresponding to C terminal residues of Streptomyces Bacteriophage PhiC31 Integrase.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurity >90%
Our Abpromise guarantee covers the use of ab93248 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 67 kDa. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.|
|ELISA||1/2000 - 1/5000.|
RelevanceIntegrase is a site-specific recombinase. Integrase contains a Ser_Recombinase domain and belongs to the Serine Recombinase family. A DNA binding domain may be present either N- or C-terminal to the catalytic domain. These enzymes perform site-specific recombination of DNA molecules by a concerted, four-strand cleavage and rejoining. The site-specific integrase from bacteriophage phiC31 functions in mammalian cells and is being applied for genetic engineering, including gene therapy. The phiC31 integrase catalyzes precise, unidirectional recombination between its 30-40-bp attP and attB recognition sites. In mammalian cells, the enzyme also mediates integration of plasmids bearing attB into native sequences that have partial sequence identity with attP, termed pseudo attP sites.
- int integrase antibody
ab93248 has been referenced in 1 publication.
- Fu L et al. The prokaryotic Argonaute proteins enhance homology sequence-directed recombination in bacteria. Nucleic Acids Res N/A:N/A (2019). PubMed: 30698806