Overview

  • Product name

    Anti-Bacteriophage PhiC31 Integrase antibody
  • Description

    Rabbit polyclonal to Bacteriophage PhiC31 Integrase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Other species
  • Immunogen

    Synthetic peptide corresponding to C terminal residues of Streptomyces Bacteriophage PhiC31 Integrase.

Properties

Applications

Our Abpromise guarantee covers the use of ab93248 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 67 kDa. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
ELISA 1/2000 - 1/5000.

Target

  • Relevance

    Integrase is a site-specific recombinase. Integrase contains a Ser_Recombinase domain and belongs to the Serine Recombinase family. A DNA binding domain may be present either N- or C-terminal to the catalytic domain. These enzymes perform site-specific recombination of DNA molecules by a concerted, four-strand cleavage and rejoining. The site-specific integrase from bacteriophage phiC31 functions in mammalian cells and is being applied for genetic engineering, including gene therapy. The phiC31 integrase catalyzes precise, unidirectional recombination between its 30-40-bp attP and attB recognition sites. In mammalian cells, the enzyme also mediates integration of plasmids bearing attB into native sequences that have partial sequence identity with attP, termed pseudo attP sites.
  • Database links

    • Alternative names

      • int integrase antibody

    References

    This product has been referenced in:

    • Fu L  et al. The prokaryotic Argonaute proteins enhance homology sequence-directed recombination in bacteria. Nucleic Acids Res N/A:N/A (2019). Read more (PubMed: 30698806) »
    See 1 Publication for this product

    Customer reviews and Q&As

    Answer

    Thank you for your enquiry regarding ab93248.
    The immunogen used to raise this antibody was a recombinant fragment. Currently, this product is not commercially available.
    Apologies!
    If you need any further assistance in the future, please do not hesitate to contact me.

    Read More

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