Bad peptide (ab171725)

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Description

Associated products

Specifications

Our Abpromise guarantee covers the use of ab171725 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking - Blocking peptide for Anti-Bad (phospho S112) antibody [EPR1891(2)] (ab129192)

  • Form

    Liquid

  • Additional notes

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

General Info

  • Alternative names

    • AI325008
    • BAD
    • BAD_HUMAN
    • BBC 2
    • BBC2
    • BBC6
    • Bcl 2 Antagonist of Cell Death
    • Bcl 2 Binding Component 6
    • BCL X / BCL 2 Binding Protein
    • BCL X Binding Protein
    • Bcl XL/Bcl 2 Associated Death Promoter
    • Bcl-2-binding component 6
    • Bcl-2-like protein 8
    • Bcl-XL/Bcl-2-associated death promoter
    • Bcl2 antagonist of cell death
    • BCL2 antagonist of cell death protein
    • BCL2 associated agonist of cell death
    • Bcl2 Associated Death Promoter
    • BCL2 binding component 6
    • BCL2 binding protein
    • Bcl2 Like 8 Protein
    • Bcl2-L-8
    • BCL2L8
    • Proapoptotic BH3 Only Protein
    see all

  • Function

    Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.

  • Tissue specificity

    Expressed in a wide variety of tissues.

  • Sequence similarities

    Belongs to the Bcl-2 family.

  • Domain

    Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.

  • Post-translational
    modifications

    Phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation. Ser-75 is phosphorylated by AKT/PKB, protein kinase A and PIM2.

  • Cellular localization

    Mitochondrion outer membrane. Cytoplasm. Upon phosphorylation, locates to the cytoplasm.
  • Information by UniProt

References

This product has been referenced in:

  • Hou Y  et al. Effect of cytokine-induced killer cells combined with dendritic cells on the survival rate and expression of 14-3-3? and p-Bad proteins in Lewis lung cancer cell lines. Oncol Lett 16:1815-1820 (2018). Read more (PubMed: 30008870) »
See 1 Publication for this product

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