Key features and details
- Rabbit polyclonal to Bad (phospho S128)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG
Product nameAnti-Bad (phospho S128) antibody
See all Bad primary antibodies
DescriptionRabbit polyclonal to Bad (phospho S128)
SpecificityThis phophorylation site is homologous to that of Ser129 in Rat.
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat
- Mouse brain and heart lysates. Rat kidney tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab216829 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/1000. Predicted molecular weight: 18 kDa.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionPromotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.
Tissue specificityExpressed in a wide variety of tissues.
Sequence similaritiesBelongs to the Bcl-2 family.
DomainIntact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
modificationsPhosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation. Ser-75 is phosphorylated by AKT/PKB, protein kinase A and PIM2.
Cellular localizationMitochondrion outer membrane. Cytoplasm. Upon phosphorylation, locates to the cytoplasm.
- Information by UniProt
- AI325008 antibody
- BAD antibody
- BAD_HUMAN antibody
Immunohistochemical analysis of formalin-fixed paraffin-embedded rat kidney tissue, labeling Bad using ab216829 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
All lanes : Anti-Bad (phospho S128) antibody (ab216829) at 1/200 dilution
Lane 1 : mouse brain lysate
Lane 2 : mouse heart lysate
All lanes : Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/3000 dilution
Developed using the ECL technique.
Predicted band size: 18 kDa
Observed band size: 18 kDa
ab216829 has been referenced in 1 publication.
- Wu DM et al. Inhibition of microRNA-200a Upregulates the Expression of Striatal Dopamine Receptor D2 to Repress Apoptosis of Striatum via the cAMP/PKA Signaling Pathway in Rats with Parkinson's Disease. Cell Physiol Biochem 51:1600-1615 (2018). PubMed: 30497067