Overview

  • Product name
    Anti-Bad (phospho S136) antibody
    See all Bad primary antibodies
  • Description
    Rabbit polyclonal to Bad (phospho S136)
  • Host species
    Rabbit
  • Specificity
    Ab28824 recognises Ser 136 phosphorylated Bad.
  • Tested applications
    Suitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic phospho peptide designed around the phosphorylation site of Serine 136 of mouse BAD/ Serine 99 of human Bad.

  • Positive control
    • Recombinant Human eIF4B protein (ab114511) can be used as a positive control in WB. IHC-P: breast carcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab28824 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000.
IHC-P Use at an assay dependent concentration.
WB 1/500 - 1/1000. Predicted molecular weight: 19 kDa.

Target

  • Function
    Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.
  • Tissue specificity
    Expressed in a wide variety of tissues.
  • Sequence similarities
    Belongs to the Bcl-2 family.
  • Domain
    Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
  • Post-translational
    modifications
    Phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation. Ser-75 is phosphorylated by AKT/PKB, protein kinase A and PIM2.
  • Cellular localization
    Mitochondrion outer membrane. Cytoplasm. Upon phosphorylation, locates to the cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI325008 antibody
    • BAD antibody
    • BAD_HUMAN antibody
    • BBC 2 antibody
    • BBC2 antibody
    • BBC6 antibody
    • Bcl 2 Antagonist of Cell Death antibody
    • Bcl 2 Binding Component 6 antibody
    • BCL X / BCL 2 Binding Protein antibody
    • BCL X Binding Protein antibody
    • Bcl XL/Bcl 2 Associated Death Promoter antibody
    • Bcl-2-binding component 6 antibody
    • Bcl-2-like protein 8 antibody
    • Bcl-XL/Bcl-2-associated death promoter antibody
    • Bcl2 antagonist of cell death antibody
    • BCL2 antagonist of cell death protein antibody
    • BCL2 associated agonist of cell death antibody
    • Bcl2 Associated Death Promoter antibody
    • BCL2 binding component 6 antibody
    • BCL2 binding protein antibody
    • Bcl2 Like 8 Protein antibody
    • Bcl2-L-8 antibody
    • BCL2L8 antibody
    • Proapoptotic BH3 Only Protein antibody
    see all

Images

  • Immunohistochemical analysis of paraffin embedded breast carcinoma, using ab28824. Left: Untreated. Right: Treated with synthesized phosphopeptide.
  • All lanes : Anti-Bad (phospho S136) antibody (ab28824) at 1/500 dilution

    Lane 1 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
    Lane 2 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size: 19 kDa
    Observed band size: 26 kDa
    why is the actual band size different from the predicted?

References

This product has been referenced in:
  • Grundy M  et al. Early changes in rpS6 phosphorylation and BH3 profiling predict response to chemotherapy in AML cells. PLoS One 13:e0196805 (2018). Read more (PubMed: 29723246) »
  • Wang T  et al. Mst1 participates in the atherosclerosis progression through macrophage autophagy inhibition and macrophage apoptosis enhancement. J Mol Cell Cardiol 98:108-116 (2016). WB ; Mouse . Read more (PubMed: 27496379) »
See all 6 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Oral squamous cell carcinoma)
Permeabilization
Yes - PBS containing 0,25% Triton X-100
Specification
Oral squamous cell carcinoma
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 24°C
Fixative
Paraformaldehyde

87andressaduarte@gmail.com

Verified customer

Submitted Sep 29 2015

Answer

Thank you for your enquiry and interest in our products.

I am sorry to confirm that because we carry over 70,000 products, it is regrettably not feasible for us to keep small sample sizes to send to customers.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

If the product is to be used in an untested species or application, you may be eligible for our testing discount program if the antibody has not yet been purchased. Please do not hesitate to contact me again by replying to this email prior to purchase and I will be pleased to send you more information.

With regards to which antibodies you would like to use, do you already have product catalog code numbers? If you would like some assistance to find suitable antibodies, I would appreciate if you are able to provide information on the application and species you will be testing. I can then find some suitably tested antibodies covered by our 6 month guarantee for your experiments.

I hope this information is helpful. I look forward to hearing from you with the requested information.

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Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement (ab1893) with the order number XXXXXXX. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.  

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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